2. Academic Validation
  3. Melatonin improves the quality of rotenone-exposed mouse oocytes through association with histone modifications

Melatonin improves the quality of rotenone-exposed mouse oocytes through association with histone modifications

  • Ecotoxicol Environ Saf. 2023 Jun 30;262:115186. doi: 10.1016/j.ecoenv.2023.115186.
Xiaofei Wang 1,  Huiying Li 2,  Hongbei Mu 3,  Shaozhe Zhang 4,  Yuanyuan Li 3,  Xiaotao Han 5,  Ling Zhang 6,  Wenpei Xiang 7
Affiliations

Affiliations

  • 1 Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; The First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
  • 2 The First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
  • 3 Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • 4 Wuhan National High Magnetic Field Center, Huazhong University of Science and Technology, Wuhan, China.
  • 5 Wuhan National High Magnetic Field Center, Huazhong University of Science and Technology, Wuhan, China. Electronic address: xthan@mail.hust.edu.cn.
  • 6 Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: Zhling312@163.com.
  • 7 Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: wpxiang2010@hust.edu.cn.
PMID: 37393821 DOI: 10.1016/j.ecoenv.2023.115186
Abstract

Rotenone, an insecticide that inhibits mitochondrial complex I and generates oxidative stress, is responsible for neurological disorders and affects the female reproductive system. However, the underlying mechanism is not fully understood. Melatonin, a potential free-radical scavenger, has been shown to protect the reproductive system from oxidative damage. In this study, we investigated the impact of rotenone on mouse oocyte quality and evaluated the protective effect of melatonin on oocytes exposed to rotenone. Our results showed that rotenone impaired mouse oocyte maturation and early embryo cleavage. However, melatonin prevented these negative effects by ameliorating rotenone-induced mitochondrial dysfunction and dynamic imbalance, intracellular CA2+ homeostasis damage, ER stress, early Apoptosis, meiotic spindle formation disruption, and aneuploidy in oocytes. Additionally, RNA Sequencing analysis showed that rotenone exposure changed the expression of multiple genes involved in histone methylation and acetylation modifications that result in mouse meiotic defects. However, melatonin partially rescued these defects. These findings suggest that melatonin has protective effects against rotenone-induced mouse oocyte defects.

Keywords

ER stress; Histone modification; Melatonin; Mitochondrial dysfunction; Rotenone.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z