2. Academic Validation
  3. Characterization of two O-methyltransferases involved in the biosynthesis of O-methylated catechins in tea plant

Characterization of two O-methyltransferases involved in the biosynthesis of O-methylated catechins in tea plant

  • Nat Commun. 2023 Aug 21;14(1):5075. doi: 10.1038/s41467-023-40868-9.
Ji-Qiang Jin # 1,  Fu-Rong Qu # 1,  Huisi Huang # 2,  Qing-Shuai Liu # 1,  Meng-Yuan Wei # 1,  Yuee Zhou 2,  Ke-Lin Huang 3,  Zhibo Cui 2,  Jie-Dan Chen 1,  Wei-Dong Dai 1,  Li Zhu 1,  Ming-Zhe Yao 4,  Zhi-Min Zhang 5,  Liang Chen 6
Affiliations

Affiliations

  • 1 Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs; Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China.
  • 2 College of Pharmacy, Jinan University, Guangzhou, 510632, China.
  • 3 State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, 230036, China.
  • 4 Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs; Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China. yaomz@tricaas.com.
  • 5 College of Pharmacy, Jinan University, Guangzhou, 510632, China. 13632107756@163.com.
  • 6 Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs; Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China. liangchen@tricaas.com.
  • # Contributed equally.
PMID: 37604798 DOI: 10.1038/s41467-023-40868-9
Abstract

Tea is known for having a high catechin content, with the main component being (-)-epigallocatechin gallate (EGCG), which has significant bioactivities, including potential anti-cancer and anti-inflammatory activity. The poor intestinal stability and permeability of EGCG, however, undermine these health-improving benefits. O-methylated EGCG derivatives, found in a few tea cultivars in low levels, have attracted considerable interest due to their increased bioavailability. Here, we identify two O-methyltransferases from tea plant: CsFAOMT1 that has a specific O-methyltransferase activity on the 3''-position of EGCG to generate EGCG3''Me, and CsFAOMT2 that predominantly catalyzes the formation of EGCG4″Me. In different tea tissues and germplasms, the transcript levels of CsFAOMT1 and CsFAOMT2 are strongly correlated with the amounts of EGCG3''Me and EGCG4''Me, respectively. Furthermore, the crystal structures of CsFAOMT1 and CsFAOMT2 reveal the key residues necessary for 3''- and 4''-O-methylation. These findings may provide guidance for the future development of tea cultivars with high O-methylated catechin content.

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