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  3. Fructus Lycii and Salvia miltiorrhiza Bunge extract attenuate oxidative stress-induced photoreceptor ferroptosis in retinitis pigmentosa

Fructus Lycii and Salvia miltiorrhiza Bunge extract attenuate oxidative stress-induced photoreceptor ferroptosis in retinitis pigmentosa

  • Biomed Pharmacother. 2023 Sep 21;167:115547. doi: 10.1016/j.biopha.2023.115547.
Yijing Yang 1,  Ying Wang 2,  Ying Deng 2,  Jing Lu 2,  Li Xiao 3,  Jie Li 2,  Yasha Zhou 3,  Fujiao Nie 4,  Xiangdong Chen 5,  Jun Peng 1,  Hanyu Tan 6,  Yuhui Qin 7,  Qinghua Peng 8
Affiliations

Affiliations

  • 1 Hunan University of Chinese Medicine, Changsha 410208, China; Institute of Ophthalmology and Otolaryngoloy of Chinese Medicine, Changsha 410208, China; Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha 410208, China.
  • 2 Hunan University of Chinese Medicine, Changsha 410208, China; Institute of Ophthalmology and Otolaryngoloy of Chinese Medicine, Changsha 410208, China.
  • 3 Hunan University of Chinese Medicine, Changsha 410208, China.
  • 4 Hunan University of Chinese Medicine, Changsha 410208, China; Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha 410208, China.
  • 5 Hunan University of Chinese Medicine, Changsha 410208, China; Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha 410208, China; Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China.
  • 6 Hunan University of Chinese Medicine, Changsha 410208, China; Institute of Ophthalmology and Otolaryngoloy of Chinese Medicine, Changsha 410208, China; Yueyang Hospital Affiliated to Hunan University of Traditional Chinese Medicine, Yueyang 414000, China.
  • 7 Hunan University of Chinese Medicine, Changsha 410208, China; Institute of Ophthalmology and Otolaryngoloy of Chinese Medicine, Changsha 410208, China; Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha 410208, China; Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China.
  • 8 Hunan University of Chinese Medicine, Changsha 410208, China; Institute of Ophthalmology and Otolaryngoloy of Chinese Medicine, Changsha 410208, China; Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha 410208, China. Electronic address: pengqinghua@hnucm.edu.cn.
PMID: 37741257 DOI: 10.1016/j.biopha.2023.115547
Abstract

Aim of the study: To assess the impact of Fructus Lycii and Salvia miltiorrhiza Bunge extract (FSE) on retinitis pigmentosa (RP) and to explore the mechanisms by which FSE can prevent oxidative stress-induced photoreceptor Ferroptosis in RP.

Methods: Hydrogen peroxide(H2O2) was used to induce oxidative stress in 661 W cells, which were then examined using flow cytometry and Enzyme linked immunosorbent assay (ELISA). Changes in mitochondria were observed by using an electron microscope to characterize the Ferroptosis of the cells. The protective effect of FSE on the retina function and structure of rd10 mice was evaluated using histopathological examination, fundus photographs, and electroretinography (ERG). Protein expression levels of Tumor Protein p53 (P53), Solute Carrier Family 7 Member 11 (SLC7A11), Glutathione Peroxidase 4 (GPX4), Arachidonate-12-Lipoxygenase (ALOX12), and Dipeptidyl Peptidase 4 (DPP4) were evaluated by Western blot assays in Vivo and in Vitro.

Results: H2O2-induced 661 W cells increased oxidative stress products and P53 and ALOX12, decreasing the expression of SLC7A11, GPX4, and DPP4. GPX4 activator does not reduce Reactive Oxygen Species (ROS) generation and has little effect on Ferroptosis. Fer-1 and FSE attenuate ROS generation and inhibit Ferroptosis of photoreceptors in RP via inhibited P53 expression and increased SLC7A11 and GPX4 expression.

Conclusion: FSE may be available in clinical therapeutics to alleviating RP and the mechanism by which inhibits Ferroptosis of photoreceptors following oxidative stress via the P53/ SLC7A11 pathway.

Keywords

Ferroptosis; Fructus Lycii; Oxidative stress; Retinitis Pigmentosa; Salvia miltiorrhiza Bunge.

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