2. Academic Validation
  3. Site-selected in situ polymerization for living cell surface engineering

Site-selected in situ polymerization for living cell surface engineering

  • Nat Commun. 2023 Nov 10;14(1):7285. doi: 10.1038/s41467-023-43161-x.
Yihong Zhong 1,  Lijia Xu 1,  Chen Yang 1,  Le Xu 2,  Guyu Wang 1,  Yuna Guo 1,  Songtao Cheng 1,  Xiao Tian 3,  Changjiang Wang 3,  Ran Xie 3 4,  Xiaojian Wang 5,  Lin Ding 6 7,  Huangxian Ju 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
  • 2 Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, 211816, China.
  • 3 State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
  • 4 Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing, 210023, China.
  • 5 Institute of Advanced Synthesis, School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, 211816, China. ias_xjwang@njtech.edu.cn.
  • 6 State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China. dinglin@nju.edu.cn.
  • 7 Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing, 210023, China. dinglin@nju.edu.cn.
PMID: 37949881 DOI: 10.1038/s41467-023-43161-x
Abstract

The construction of polymer-based mimicry on cell surface to manipulate cell behaviors and functions offers promising prospects in the field of biotechnology and cell therapy. However, precise control of polymer grafting sites is essential to successful implementation of biomimicry and functional modulation, which has been overlooked by most current research. Herein, we report a biological site-selected, in situ controlled radical polymerization platform for living cell surface engineering. The method utilizes metabolic labeling techniques to confine the growth sites of Polymers and designs a Fenton-RAFT polymerization technique with cytocompatibility. Polymers grown at different sites (glycans, proteins, lipids) have different membrane retention time and exhibit differential effects on the recognition behaviors of cellular glycans. Of particular importance is the achievement of in situ copolymerization of glycomonomers on the outermost natural glycan sites of cell membrane, building a biomimetic glycocalyx with distinct recognition properties.

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