2. Academic Validation
  3. Detection of glucose transporter 1 in living cells for assessment of tumor development and therapy using an electrochemical biosensor

Detection of glucose transporter 1 in living cells for assessment of tumor development and therapy using an electrochemical biosensor

  • Biosens Bioelectron. 2023 Nov 8:244:115820. doi: 10.1016/j.bios.2023.115820.
Kaijing Wang 1,  Congcong Zhang 2,  Han Zhou 2,  Hongshuai Wei 2,  Liping Yin 2,  Tingting Zhang 2,  Yunqing Zhi 3,  Jun Zhou 2,  Bingkai Han 4,  Zhenguo Zhang 5,  Xin Du 6
Affiliations

Affiliations

  • 1 Department of Hepatobiliary Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200092, China.
  • 2 Center for Cell Structure and Function, Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, Jinan, Shandong, 250014, China.
  • 3 Department of Assisted Reproductive Medicine, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, 200092, China.
  • 4 Tianjin Key Laboratory of Exercise Physiology and Sports Medicine, Institute of Sport, Exercise & Health, Tianjin University of Sport, Tianjin, 300381, China.
  • 5 Center for Cell Structure and Function, Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, Jinan, Shandong, 250014, China. Electronic address: zhangzg@sdnu.edu.cn.
  • 6 Center for Cell Structure and Function, Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, Jinan, Shandong, 250014, China. Electronic address: xdu@sdnu.edu.cn.
PMID: 37952321 DOI: 10.1016/j.bios.2023.115820
Abstract

The expression level of glucose transporter 1 (GLUT-1) is highly correlated with tumor malignancy, making it a promising therapeutic target for Cancer treatment. The detection of GLUT-1 expression level is significant for Cancer discovery and valuating the efficacy of drug treatments. However, current methods for GLUT-1 detection primarily rely on traditional techniques. Therefore, the development of anon-destructive in vivo monitoring system would be invaluable for assessing GLUT-1 expression and tumor responses to various drugs. In this study, an electrochemical platform for detection of GLUT-1 on living cells was established using reduced graphene oxide-multi-wall carbon nanotube composite (rGO-MWCNT) as a conductive coating and toluidine blue O (TBO)-graphene-gold nanoparticle-GLUT-1 antibody as the electrochemical probe. The sensor demonstrated excellent performance in detecting GLUT-1 on cells with a linear range of 10 - 105 cells/mL, good stability and selectivity. The sensor successfully detected GLUT-1 expressions in multiple tumor cell types, including those treated with siRNA or drugs, and the results were consistent with those obtained from traditional methods such as flow cytometry, western blotting, and immunofluorescence. The sensor is promising in evaluating the malignant level of tumor cells, distinguishing glucose uptake pathways in tumor cells, reducing medical costs, and facilitating the translation of electrochemical sensing technology to the clinical settings.

Keywords

Electrochemical sensor; Glucose transporter 1; Glucose uptake; Living tumor cell.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-100017
    99.67%, GLUT1抑制剂
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