1. Academic Validation
  2. Deficiency of aldehyde dehydrogenase 2 aggravates ethanol-induced cytotoxicity in N2a cells via CaMKII/Drp1-mediated mitophagy

Deficiency of aldehyde dehydrogenase 2 aggravates ethanol-induced cytotoxicity in N2a cells via CaMKII/Drp1-mediated mitophagy

  • Food Chem Toxicol. 2023 Nov 13:114129. doi: 10.1016/j.fct.2023.114129.
Tingting Yan 1 Jiyang Chen 1 Yalin Wang 1 Yinuo Wang 1 Yuanqingzhi Zhang 1 Yan Zhao 2
Affiliations

Affiliations

  • 1 Department of Bioengineering, Harbin Institute of Technology, Weihai 264209, Shandong, China.
  • 2 Department of Bioengineering, Harbin Institute of Technology, Weihai 264209, Shandong, China. Electronic address: zhaoyan@hitwh.edu.cn.
Abstract

Chronic alcohol abuse causes brain damage and has been associated with an increased risk of Alzheimer's disease. The toxic metabolite of alcohol, acetaldehyde, which is converted to acetate by aldehyde dehydrogenase 2 (ALDH2), has been shown to induce excessive mitochondrial fragmentation and dysfunction leading to neurotoxicity. However, it is still unclear how alcohol affects mitochondrial function in ALDH2-deficient cells. The present study investigated the association between abnormal mitochondrial dynamics, Mitophagy and cytotoxicity in ALDH2-deficient N2a cells treated with ethanol. It was found that ethanol induced dynamin-related protein 1 (Drp1)-mediated mitochondrial fragmentation and impaired mitochondrial function, causing excessive Mitophagy and cytotoxicity in ALDH2-deficient N2a cells while inducing CA2+ influx and activating CA2+/calmodulin-dependent protein kinase II (CaMKII). Inhibition of CA2+ overload or CaMKII activation prevented Drp1 phosphorylation and ameliorated ethanol-induced Mitophagy and cytotoxicity, indicating that CA2+-dependent CaMKII activation was critical for mediating Drp1-dependent excessive mitochondrial fission and Mitophagy in ALDH2-deficient N2a cells. The results of the present study suggested that prevention of intracellular CA2+ overload might be beneficial for preventing neurotoxicity associated with alcohol abuse in individuals with defective ALDH2.

Keywords

ALDH2; CaMKII; Drp1; Ethanol; Mitochondrial dysfunction; Mitophagy.

Figures
Products