2. Academic Validation
  3. Jiedu Fuzheng decoction improves the proliferation, migration, invasion and EMT of non-small cell lung cancer via the Wnt/β-catenin pathway

Jiedu Fuzheng decoction improves the proliferation, migration, invasion and EMT of non-small cell lung cancer via the Wnt/β-catenin pathway

  • Cell Div. 2023 Dec 16;18(1):22. doi: 10.1186/s13008-023-00105-7.
Fang Fang # 1,  Xiaowei Jin # 2,  Jinming Meng 3,  Jiaqi He 3,  Jiaxiao Wang 1,  Changhong Wang 1,  Sheng Xie 4,  Wei Shi 5
Affiliations

Affiliations

  • 1 The Second Ward of Oncology, The First Affiliated Hospital of Guangxi University of Chinese Medicine, No. 327, Xianhu Avenue, Qingxiu District, Nanning, 530001, Guangxi Zhuang Autonomous Region, People's Republic of China.
  • 2 Department of Traditional Chinese Medicine, Yunnan Cancer Hospital, Kunming, 650018, Yunnan, People's Republic of China.
  • 3 Guangxi University of Chinese Medicine, Nanning, 530001, Guangxi Zhuang Autonomous Region, People's Republic of China.
  • 4 Preventive Treatment of Disease Center, The First Affiliated Hospital of Guangxi University of Chinese Medicine, No. 89-9, Dongge Road, Qingxiu District, Nanning, 530001, Guangxi Zhuang Autonomous Region, People's Republic of China. xsh6566@163.com.
  • 5 The Second Ward of Oncology, The First Affiliated Hospital of Guangxi University of Chinese Medicine, No. 327, Xianhu Avenue, Qingxiu District, Nanning, 530001, Guangxi Zhuang Autonomous Region, People's Republic of China. shiwei023009@qq.com.
  • # Contributed equally.
PMID: 38104091 DOI: 10.1186/s13008-023-00105-7
Abstract

Objectives: This study aimed to investigate the effect of Jiedu Fuzheng decoction (JFD) in non-small cell lung Cancer (NSCLC) and its potential therapeutic mechanism.

Results: We prepared JFD-medicated serum from rats and treated NSCLC cells (A549 and NCI-H1650) with 0.5, 1, and 2 mg/mL JFD-medicated serum. CCK-8 and colony formation assays were used to detect cell proliferation. Transwell assays showed that JFD attenuated cell migration and invasion. JFD and SKL2001 (Wnt/β-catenin activator) were simultaneously used to treat NSCLC cells to verify that JFD regulated the biological behavior of NSCLC via Wnt/β-catenin signaling. It was found that 2 mg/mL JFD had the most significant effect on the activity of NSCLC cells. JFD attenuated proliferation and metastasis but increased the proportion of apoptotic cells. At the same time, JFD downregulated N-Cadherin, vimentin and β-catenin protein expression in Cancer cells. SKL2001 could restore the improvement of JFD on proliferation, metastasis and Apoptosis.

Conclusion: This study confirmed that JFD suppressed the occurrence and development of NSCLC by regulating Wnt/β-catenin signaling and provided a novel therapeutic scheme for NSCLC.

Keywords

Invasion; Jiedu Fuzheng decoction; Lung cancer; Migration; Wnt/β-catenin.

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