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  3. Programmable protein expression using a genetically encoded m6A sensor

Programmable protein expression using a genetically encoded m6A sensor

  • Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-01978-3.
Bahjat F Marayati 1,  Matthew G Thompson 2,  Christopher L Holley 3 4,  Stacy M Horner 2 3,  Kate D Meyer 5 6
Affiliations

Affiliations

  • 1 Department of Biochemistry, Duke University School of Medicine, Durham, NC, USA.
  • 2 Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA.
  • 3 Department of Medicine, Duke University School of Medicine, Durham, NC, USA.
  • 4 Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, USA.
  • 5 Department of Biochemistry, Duke University School of Medicine, Durham, NC, USA. kate.meyer@duke.edu.
  • 6 Department of Neurobiology, Duke University School of Medicine, Durham, NC, USA. kate.meyer@duke.edu.
PMID: 38168988 DOI: 10.1038/s41587-023-01978-3
Abstract

The N6-methyladenosine (m6A) modification is found in thousands of cellular mRNAs and is a critical regulator of gene expression and cellular physiology. m6A dysregulation contributes to several human diseases, and the m6A methyltransferase machinery has emerged as a promising therapeutic target. However, current methods for studying m6A require RNA isolation and do not provide a real-time readout of mRNA methylation in living cells. Here we present a genetically encoded m6A sensor (GEMS) technology, which couples a fluorescent signal with cellular mRNA methylation. GEMS detects changes in m6A caused by pharmacological inhibition of the m6A methyltransferase, giving it potential utility for drug discovery efforts. Additionally, GEMS can be programmed to achieve m6A-dependent delivery of custom protein payloads in cells. Thus, GEMS is a versatile platform for m6A sensing that provides both a simple readout for m6A methylation and a system for m6A-coupled protein expression.

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