Green synthesis of Zn2+ nanocarriers from Auricularia auricula fermentation broth with excellent antioxidant activity

Nanoparticles (NPs) possessing nanoscale dimensions and remarkable antioxidant activity were synthesized via a green hydrothermal method utilizing Auricularia auricula fermentation broth, referred to as AFNPs. The functional groups on the surface of the AFNPs significantly contributed to the formation of AFNPs-Zn²⁺. The AFNPs-Zn²⁺ appeared a zinc retention rate of 40.80 % after gastrointestinal digestion. When compared to typical zinc supplements, AFNPs-Zn²⁺ did not exhibit visible cytotoxicity or hemolysis. Furthermore, AFNPs-Zn²⁺ demonstrated the ability to mitigate cell damage resulting from zinc deficiency. In vivo experiments showed that AFNPs-Zn²⁺ were mainly observed in the stomach, intestine, kidney, and testis after oral administration. In vivo distribution experiments indicated predominant presence of AFNPs-Zn²⁺ in the stomach, intestine, kidney, and testis following oral administration. This study highlights the potential for Auricularia auricula NPs to serve as the efficient, stable, and safe nanocarriers for Zn²⁺.

Auricularia auricula fermentation broth; Food-borne nanoparticles; Green synthesis; Zn(2+) nanocarriers.