1. Academic Validation
  2. Human seminal extracellular vesicles enhance endometrial receptivity through leukemia inhibitory factor

Human seminal extracellular vesicles enhance endometrial receptivity through leukemia inhibitory factor

  • Endocrinology. 2024 Mar 22:bqae035. doi: 10.1210/endocr/bqae035.
Hanshu Wang 1 Yu Lin 2 Rongrong Chen 1 Yu Zhu 3 Hongqiang Wang 1 Shengxian Li 1 Lei Yu 1 Kaishu Zhang 1 Yujie Liu 1 Tao Jing 1 Fei Sun 4 5
Affiliations

Affiliations

  • 1 Department of Andrology, the Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong Province, China.
  • 2 International Peace Maternity & Child Health Hospital, Shanghai Municipal Key Clinical Specialty, Institute of Embryo-Fetal Original Adult Disease, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.
  • 3 Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, 201204, China.
  • 4 Department of Urology and Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310020 , Zhejiang, China.
  • 5 School of Basic Medical Sciences, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan 750004, China.
Abstract

Seminal extracellular vesicles (EVs) contain different subgroups which have diverse effects on sperm function. However, the effect of seminal EVs-especially its subgroups, on the endometrial receptivity was largely unknown. Here, we found that the seminal EVs could be divided into high-density EV (EV-H)、medium density EV (EV-M) and low-density EV (EV-L), after purification using iodixanol. Then we demonstrated that EV-H could promote the expression and secretion of leukemia inhibitor factor (LIF) in human endometrial cells. In EV-H-treated endometrial cells, we identified 1274 differentially expressed genes (DEGs). DEGs were enriched in cell adhesion and Akt, STAT3 pathways. Therefore, we illustrated that EV-H enhanced the adhesion of human choriocarcinoma JAr cell spheroids to endometrial cells through the LIF-STAT3 pathway. Collectively, our findings indicated that seminal EV-H could regulate endometrial receptivity through LIF pathway, which would provide novel insights into male fertility.

Keywords

LIF; adhesion; endometrial receptivity; integrin; seminal extracellular vesicles.

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  • HY-120142
    99.09%, LIFR抑制剂