2. Academic Validation
  3. In vitro assessment of ferroptosis of cells exposed to cigarette smoke aerosol using a self-designed on-chip evaluation system based on gas-liquid dual-dimensional exposure

In vitro assessment of ferroptosis of cells exposed to cigarette smoke aerosol using a self-designed on-chip evaluation system based on gas-liquid dual-dimensional exposure

  • Talanta. 2024 Jun 1:277:126352. doi: 10.1016/j.talanta.2024.126352.
Zezhi Li 1,  Xiang Li 2,  Kejian Liu 3,  Junwei Zhao 4,  Pingping Shang 3,  Chenfeng Hua 3,  Junwei Guo 3,  Fuwei Xie 3,  Jianping Xie 5
Affiliations

Affiliations

  • 1 Beijing Life Science Academy, Beijing, 102209, PR China; Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001, PR China; Beijing Technology and Business University, Beijing, 100048, PR China.
  • 2 Beijing Life Science Academy, Beijing, 102209, PR China; Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001, PR China. Electronic address: lixiang79ben@sina.com.
  • 3 Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001, PR China.
  • 4 Beijing Life Science Academy, Beijing, 102209, PR China; Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001, PR China.
  • 5 Beijing Life Science Academy, Beijing, 102209, PR China; Key Laboratory of Tobacco Chemistry, Zhengzhou Tobacco Research Institute of CNTC, No. 2 Fengyang Street, Zhengzhou, 450001, PR China. Electronic address: ztridicp@126.com.
PMID: 38838566 DOI: 10.1016/j.talanta.2024.126352
Abstract

Aerosol pollutants significantly cause health concerns. Herein, we established an original real-time aerosol exposure system that used a self-designed bionic-lung microfluidic chip. The chip features a 4 × 4 intersecting array within gas and liquid layers, creating 16 distinct microenvironments. A membrane situated between the layers offers attachment for cells and establishes a gas-liquid interface. This design provides a reliable screening capacity for investigating the biological effects of aerosol exposure in vitro by manipulating the gas and/or liquid conditions. Using this system, we validated that cigarette smoke (CS) aerosol triggered a concentration- and time-dependent reduction in cell viability and intracellular glutathione levels, accompanied by an increase in intracellular Reactive Oxygen Species and Fe2+. Furthermore, CS aerosol significantly downregulated the expression of GPX4, SLC7A11, and FTL mRNA while inducing a notable increase in that of ACSL4 mRNA. Additionally, CS aerosol markedly stimulated the release of proinflammatory cytokines. Crucially, the Ferroptosis inhibitor deferoxamine mesylate reversed these biological indicators. These results demonstrate that our novel bionic-lung chip presents a suitably achievable approach to investigate the biological effects induced by aerosol exposure.

Keywords

Aerosol exposure system; Bionic-lung chip; Ferroptosis; Multi-throughput.

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