2. Academic Validation
  3. Portable Near-Infrared to Near-Infrared Platform for Homogeneous Quantification of Biomarkers in Complex Biological Samples

Portable Near-Infrared to Near-Infrared Platform for Homogeneous Quantification of Biomarkers in Complex Biological Samples

  • ACS Nano. 2024 Jul 19. doi: 10.1021/acsnano.4c05280.
Yantao Li 1 2,  Xiaorui Mai 1,  Wenming Liu 3,  Feng Wang 1,  Shuo Yan 1,  Yu Lei 1,  Lu Chen 1,  Weikang Mai 1,  Qingwei Song 2,  Weidong Du 2,  Xukai Chen 2,  Huiru Ye 2,  Longfei Song 2,  Yu Chen 2,  Lei Zhao 2,  Zhenwei Liu 4,  Weidong Ding 5,  Pei Yu 1,  Xue Jiang 6,  Yuyi Li 7,  Jing Huang 7,  Qiuqiang Zhan 7,  Yiru Qin 8,  Chenzhong Li 9 10,  Wei Wei 2,  Tianxing Ji 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Respiratory Disease, Clinical Laboratory Medicine Department, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, P. R. China.
  • 2 MOE & Guangdong Provincial Key Laboratory of Laser Life Science, Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou 510631, P. R. China.
  • 3 Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510535, P. R. China.
  • 4 Department of Pediatrics, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, P. R. China.
  • 5 Shenzhen Highcreation Technology Co., Ltd., Shenzhen 518122, P. R. China.
  • 6 State Key Laboratory of Pharmaceutical Biotechnology, The University of Hong Kong, Hong Kong, 999077, P. R. China.
  • 7 Centre for Optical and Electromagnetic Research, Guangdong Engineering Research Centre of Optoelectronic Intelligent Information Perception, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510631, P. R. China.
  • 8 Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, P. R. China.
  • 9 Biomedical Engineering Division, Chinese University of Hong Kong (Shenzhen), Shenzhen 518172, China.
  • 10 Jvxin Tang Biotechnology Inc., Chengdu Future Medical City, Chengdu 610095, China.
PMID: 39028863 DOI: 10.1021/acsnano.4c05280
Abstract

Förster resonance energy transfer (FRET)-based homogeneous immunoassay obviates tedious washing steps and thus is a promising approach for immunoassays. However, a conventional FRET-based homogeneous immunoassay operating in the visible region is not able to overcome the interference of complex biological samples, thus resulting in insufficient detection sensitivity and poor accuracy. Here, we develop a near-infrared (NIR)-to-NIR FRET platform (Ex = 808 nm, Em = 980 nm) that enables background-free high-throughput homogeneous quantification of various biomarkers in complex biological samples. This NIR-to-NIR FRET platform is portable and easy to operate and is mainly composed of a high-performance NIR-to-NIR FRET pair based on lanthanide-doped nanoparticles (LnNPs) and a custom-made microplate reader for readout of NIR luminescence signals. We demonstrate that this NIR-to-NIR FRET platform is versatile and robust, capable of realizing highly sensitive and accurate detection of various critical biomarkers, including small molecules (morphine and 1,25-dihydroxyvitamin D), proteins (human chorionic gonadotropin), and viral particles (adenovirus) in unprocessed complex biological samples (urine, whole blood, and feces) within 5-10 min. We expect this NIR-to-NIR FRET platform to provide low-cost healthcare for populations living in resource-limited areas and be widely used in many other fields, such as food safety and environmental monitoring.

Keywords

Förster resonance energy transfer; complex biological samples; high-throughput background-free homogeneous quantification; lanthanide-doped nanoparticles; near-infrared to near-infrared.

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