2. Academic Validation
  3. A repressive H3K36me2 reader mediates Polycomb silencing

A repressive H3K36me2 reader mediates Polycomb silencing

  • Nat Commun. 2024 Aug 24;15(1):7287. doi: 10.1038/s41467-024-51789-6.
Mengting Xu 1,  Qi Zhang 1,  Huanbin Shi 2,  Zhongling Wu 1,  Wei Zhou 1,  Fucheng Lin 3,  Yanjun Kou 4,  Zeng Tao 5
Affiliations

Affiliations

  • 1 State Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
  • 2 State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China.
  • 3 State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
  • 4 State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, China. kouyanjun@caas.cn.
  • 5 State Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou, China. taozeng@zju.edu.cn.
PMID: 39179589 DOI: 10.1038/s41467-024-51789-6
Abstract

In Animals, evolutionarily conserved Polycomb repressive complex 2 (PRC2) catalyzes histone H3 lysine 27 trimethylation (H3K27me3) and PRC1 functions in recruitment and transcriptional repression. However, the mechanisms underlying H3K27me3-mediated stable transcriptional silencing are largely unknown, as PRC1 subunits are poorly characterized in fungi. Here, we report that in the filamentous fungus Magnaporthe oryzae, the N-terminal chromodomain and C-terminal MRG domain of Eaf3 play key roles in facultative heterochromatin formation and transcriptional silencing. Eaf3 physically interacts with Ash1, Eed, and Sin3, encoding an H3K36 methyltransferase, the core subunit of PRC2, and a histone deacetylation co-suppressor, respectively. Eaf3 co-localizes with a set of repressive Ash1-H3K36me2 and H3K27me3 loci and mediates their transcriptional silencing. Furthermore, Eaf3 acts as a histone reader for the repressive H3K36me2 and H3K27me3 marks. Eaf3-occupied regions are associated with increased nucleosome occupancy, contributing to transcriptional silencing in M. oryzae. Together, these findings reveal that Eaf3 is a repressive H3K36me2 reader and plays a vital role in Polycomb gene silencing and the formation of facultative heterochromatin in fungi.

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