2. Academic Validation
  3. A compact, versatile drug-induced splicing switch system with minimal background expression

A compact, versatile drug-induced splicing switch system with minimal background expression

  • Cell Rep Methods. 2024 Sep 16;4(9):100842. doi: 10.1016/j.crmeth.2024.100842.
Yue Chi 1,  Xuan Lu 1,  Shuangpeng Li 1,  Jinling Wang 1,  Jiahui Xi 1,  Xiaoqing Zhou 1,  Chengcheng Tang 1,  Min Chen 1,  Hui Yuan 1,  Shuo Lin 1,  Yingying Xiao 2,  Liangxue Lai 3,  Qingjian Zou 4
Affiliations

Affiliations

  • 1 Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China.
  • 2 Jiangmen Wuyi Traditional Chinese Medicine Hospital, Jiangmen 529000, China.
  • 3 Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China; CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China. Electronic address: lai_liangxue@gibh.ac.cn.
  • 4 Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, South China Institute of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen 529020, China. Electronic address: zouqj@wyu.edu.cn.
PMID: 39236714 DOI: 10.1016/j.crmeth.2024.100842
Abstract

Gene-switch techniques hold promising applications in contemporary genetics research, particularly in disease treatment and genetic engineering. Here, we developed a compact drug-induced splicing system that maintains low background using a human ubiquitin C (hUBC) promoter and optimized drug (LMI070) binding sequences based on the Xon switch system. To ensure precise subcellular localization of the protein of interest (POI), we inserted a 2A self-cleaving peptide between the extra N-terminal peptide and POI. This streamlined and optimized switch system, named miniXon2G, effectively regulated POIs in different subcellular localizations both in vitro and in vivo. Furthermore, miniXon2G could be integrated into endogenous gene loci, resulting in precise, reversible regulation of target genes by both endogenous regulators and drugs. Overall, these findings highlight the performance of miniXon2G in controlling protein expression with great potential for general applicability to diverse biological scenarios requiring precise and delicate regulation.

Keywords

CP: Biotechnology; CP: Molecular biology; Xon switch; induced splicing; miniXon2G; minimal background; self-cleaving peptide.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z