2. Academic Validation
  3. Protocol for detecting lysosome quantity and membrane permeability in acute myeloid leukemia cell lines

Protocol for detecting lysosome quantity and membrane permeability in acute myeloid leukemia cell lines

  • STAR Protoc. 2024 Sep 20;5(3):103309. doi: 10.1016/j.xpro.2024.103309.
Kexiu Huang 1,  Xinya Jiang 2,  Juan Du 3,  Hui Zeng 4
Affiliations

Affiliations

  • 1 Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China. Electronic address: huang@stu2022.jnu.edu.cn.
  • 2 Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China; Department of Hematology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
  • 3 Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, China. Electronic address: du.juan@u.nus.edu.
  • 4 Department of Hematology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong 510180, China. Electronic address: androps2011@hotmail.com.
PMID: 39269898 DOI: 10.1016/j.xpro.2024.103309
Abstract

Lysosomal function and activity are essential to support cellular adaptation to multiple stresses. For example, certain drugs can induce increased lysosomal membrane permeability to exert their anti-cancer effects. Here, we present a protocol to evaluate the lysosome alterations induced by drug treatment. We first describe the steps for inducing lysosomal alterations in cultured cells. We then show how to quantify the number of lysosomes, assess the integrity of lysosomal membranes, and determine lysosomal membrane permeabilization by using Galectin puncta assay. For complete details on the use and execution of this protocol, please refer to Jiang et al.1.

Keywords

cancer; cell biology; cell culture; flow cytometry.

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