2. Academic Validation
  3. A rapid and versatile reverse genetic approach and visualization animal models for emerging zoonotic pseudorabies virus

A rapid and versatile reverse genetic approach and visualization animal models for emerging zoonotic pseudorabies virus

  • Antiviral Res. 2024 Dec:232:106036. doi: 10.1016/j.antiviral.2024.106036.
Yu Zhang 1,  Xiangtong Li 1,  Juan Zhang 1,  Yueyue Duan 1,  Peibin Chen 1,  Lei Shi 1,  Cong Yuan 1,  Liyan Cao 1,  Maowen Sun 1,  Yating Wang 1,  Xiangyu Kong 1,  Haixue Zheng 2,  Qi Wang 3
Affiliations

Affiliations

  • 1 State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China; Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China; Chengdu National Agricultural Science and Technology Center, Chengdu, China.
  • 2 State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China. Electronic address: haixuezheng@163.com.
  • 3 State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China; Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China; Chengdu National Agricultural Science and Technology Center, Chengdu, China. Electronic address: qiwang@caas.cn.
PMID: 39522887 DOI: 10.1016/j.antiviral.2024.106036
Abstract

Pseudorabies virus (PRV), a member of the Alphaherpesvirinae subfamily and a causative pathogen of Aujeszky's disease, has a broad host range including domestic and wild Animals. PRV has been reported as a causative agent in patients with acute encephalitis in 2021, which suggests PRV might be a novel animal-origin virus in terms of zoonotic spillover and spread potential. To manage current PRV epidemics in pigs and prepare for future pandemics in Other species including humans. Fundamental techniques essential for procuring such knowledge on prevention and therapy of PRV. Here, PRV CD22 strain was isolated and phylogenetic analysis showed that PRV CD22 belongs to the current epidemic strains in China. PRV CD22 was highly lethal to mice and piglets in vivo. Moreover, a rapid and efficient system to generate recombinant PRV was constructed based on PRV CD22 genomic DNA fosmid library. Using this system, a recombinant PRV strain expressing engineered labeling protein was rescued for visualization of viral Infection in mouse model. Our study allows the generation of PRV that can be used for downstream treatment analyses. Once experimental or surveillance samples are obtained, PRV can be generated and treated efficiently based on our study.

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