2. Academic Validation
  3. Mutant PP2A Induces IGFBP2 Secretion to Promote Development of High-Grade Uterine Cancer

Mutant PP2A Induces IGFBP2 Secretion to Promote Development of High-Grade Uterine Cancer

  • Cancer Res. 2025 Feb 1;85(3):442-461. doi: 10.1158/0008-5472.CAN-24-1263.
Terrance J Haanen 3rd 1 2,  Sophie Boock 2,  Catherine G Callahan 2,  Irene Peris 2,  Kaitlin P Zawacki 2,  Brynne Raines 2 3,  Charles A Nino 2 3,  Brian Tran 2 4,  Alexis Harold 1 2,  Gabrielle Hodges Onishi 2,  Matthew Hinderman 2,  Amanda Dowdican 2,  Wei Huang 5 6,  Derek J Taylor 5 6,  Sarah E Taylor 7,  Mark W Jackson 7,  Analisa DiFeo 8 9 10,  Caitlin M O'Connor 2 8,  Goutham Narla 2 8
Affiliations

Affiliations

  • 1 Department of Cancer Biology, University of Michigan, Ann Arbor, Michigan.
  • 2 Division of Genetic Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan.
  • 3 Department of Cellular and Molecular Biology, University of Michigan, Ann Arbor, Michigan.
  • 4 Department of Pharmacology, University of Michigan, Ann Arbor, Michigan.
  • 5 Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio.
  • 6 Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio.
  • 7 Department of Pathology, Case Western Reserve University, Cleveland, Ohio.
  • 8 Rogel Cancer Center, University of Michigan Health, Ann Arbor, Michigan.
  • 9 Department of Pathology, University of Michigan, Ann Arbor, Michigan.
  • 10 Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan.
PMID: 39531506 DOI: 10.1158/0008-5472.CAN-24-1263
Abstract

Uterine serous carcinoma (USC) and uterine carcinosarcoma (UCS) tumors are uniquely aggressive, suggesting that the primary tumor is intrinsically equipped to disseminate and metastasize. Previous work identified mutational hotspots within PPP2R1A, which encodes the Aα scaffolding subunit of protein Phosphatase 2A (PP2A), a heterotrimeric serine/threonine Phosphatase. Two recurrent heterozygous PPP2R1A mutations, P179R and S256F, occur exclusively within high-grade subtypes of uterine Cancer and can drive tumorigenesis and metastasis. Elucidation of the mechanisms by which PP2A Aα mutants promote tumor development and progression could help identify therapeutic opportunities. Here, we showed that expression of these mutants in USC/UCS cell lines enhanced tumor-initiating capacity, drove a hybrid epithelial-to-mesenchymal plasticity phenotype, and elevated secretion of the tumorigenic cytokine Insulin growth factor (IGF) binding protein 2 (IGFBP2). Therapeutic targeting of the IGFBP2/IGF receptor 1 signaling axis using small molecules and genetic approaches resulted in marked tumor growth inhibition. Mechanistically, PP2A regulated IGFBP2 expression through the transcription factor, NF-κB, which harbors a B56 recognition motif. Collectively, these results identify a role for PP2A in regulating paracrine Cancer cell signaling that can be targeted to block the initiation and metastasis of high-grade uterine Cancer. Significance: Elevated IGFBP2 secretion by uterine Cancer cells with heterozygous PPP2R1A mutations supports tumor progression and confers a vulnerability to IGFBP2/IGF1R inhibition as a therapeutic approach for this highly aggressive Cancer subtype.

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