2. Academic Validation
  3. Succinate exacerbates mastitis in mice via extracellular vesicles derived from the gut microbiota: a potential new mechanism for mastitis

Succinate exacerbates mastitis in mice via extracellular vesicles derived from the gut microbiota: a potential new mechanism for mastitis

  • J Nanobiotechnology. 2024 Nov 15;22(1):712. doi: 10.1186/s12951-024-02997-1.
Min Qiu # 1 2,  Cong Ye # 1,  Xiaotong Zhao 2,  Chenyu Zou 2,  Ruibo Tang 2,  Jiaxin Xie 2,  Yiheng Liu 2,  Yubo Hu 3,  Xiaoyu Hu 2,  Naisheng Zhang 2,  Yunhe Fu 4,  Jun Wang 5,  Caijun Zhao 6
Affiliations

Affiliations

  • 1 Department of Gynecology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130033, China.
  • 2 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, China.
  • 3 Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Erdao District, 126 Sendai Street, Changchun, Jilin Province, 130033, China.
  • 4 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, China. fuyunhesky@sina.com.
  • 5 College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province, 130118, China. Junwang2004@126.com.
  • 6 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin Province, 130062, China. zhaocj2001@sina.com.
  • # Contributed equally.
PMID: 39543623 DOI: 10.1186/s12951-024-02997-1
Abstract

Background: A high grain diet causes an ecological imbalance in the gut microbiota and serves as an important endogenous trigger of mastitis in dairy cows, but the underlying mechanisms are unclear. Our previous study revealed that subacute rumen acidosis (SARA)-associated mastitis has distinct metabolic profiles in the rumen, especially a significant increase in succinate, but the role of succinate in the pathogenesis of mastitis remains unclear.

Results: Succinate treatment exacerbates low-grade endotoxemia-induced mastitis in mice. Specifically, succinate increased the production of gut microbiota-extracellular vehicles (mEVs) containing lipopolysaccharides, which can diffuse across the damaged intestinal barrier into the mammary glands. Administration of mEVs promotes mammary inflammation via activation of the TLR4/NF-κB pathway.

Conclusions: Our findings suggest that succinate promotes mastitis through the proliferation of enteric pathogens and mEVs production, suggesting a potential strategy for mastitis intervention on the basis of intestinal metabolic regulation and pathogen inhibition. The role of mEVs in interspecific communication has also been elucidated.

Keywords

Extracellular vesicles; Mastitis; Succinate; TLR4/NF-κB.

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