2. Academic Validation
  3. Deep-supercooling preservation of stem cell spheroids for chondral defect repairment

Deep-supercooling preservation of stem cell spheroids for chondral defect repairment

  • Stem Cell Reports. 2024 Dec 10;19(12):1665-1676. doi: 10.1016/j.stemcr.2024.10.008.
Jinbin Qiu 1,  Bangrui Yu 1,  Cheng Ren 1,  Tian Wang 1,  Guangjian Zhang 2,  Zhe Jian 3,  Jian Ding 4,  Feng Xu 5,  Haishui Huang 6
Affiliations

Affiliations

  • 1 The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an 710049, P.R. China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, P.R. China.
  • 2 Department of Thoracic Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710049, P.R. China; Key Laboratory of Enhanced Recovery After Surgery of Integrated Chinese and Western Medicine, Administration of Traditional Chinese Medicine of Shaanxi Province, Xi'an 710049, P.R. China.
  • 3 Department of dermatology, Xijing hospital, Fourth Military Medical University, Xi'an 710032, P.R. China.
  • 4 The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an 710049, P.R. China.
  • 5 The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an 710049, P.R. China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, P.R. China. Electronic address: fengxu@mail.xjtu.edu.cn.
  • 6 The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an 710049, P.R. China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, P.R. China. Electronic address: haishuihuang@xjtu.edu.cn.
PMID: 39577429 DOI: 10.1016/j.stemcr.2024.10.008
Abstract

Versatile mesenchymal stem cells (MSCs) play an important role in tissue engineering and regenerative medicine. MSCs in 3D spheroid have shown higher secretion and differentiation functions than suspended counterparts, and, thus, in vitro cryopreservation of MSC spheroids is an indispensable technology to bridge the spatiotemporal gaps between spheroid generation and application. Traditional cryopreservation methods are inapplicable for spheroid due to severe thermal stress, toxic cryoprotectants, and ice formation. Here, we constructed and preserved human MSC (hMSC) spheroids via deep supercooling (DSC). Spheroids were DSC preserved at -12°C without ice formation for 7 days, with higher cell viability, energy level, and chondrogenic differentiation capacity than suspended hMSCs. hMSCs embedded in spheroids have close cell-cell interactions via N-Cadherin to activate the AKT-cytochrome c-caspase anti-apoptotic cascade during DSC preservation. Finally, preserved hMSC spheroids were capable of chondrogenic differentiation and can be co-delivered with collagen to treat rat cartilage defects in vivo.

Figures
Products
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    Target
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  • HY-15534
    99.0%, 线粒体膜电位探针
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