2. Academic Validation
  3. Establishment and characterization of the novel myxofibrosarcoma cell line, SMU-MFS

Establishment and characterization of the novel myxofibrosarcoma cell line, SMU-MFS

  • Hum Cell. 2024 Dec 3;38(1):25. doi: 10.1007/s13577-024-01157-9.
Naoya Nakahashi 1 2,  Makoto Emori 3,  Kohichi Takada 4,  Yasutaka Murahashi 1,  Junya Shimizu 1,  Kazuyuki Murase 4,  Tomohide Tsukahara 2,  Shintaro Sugita 5,  Akira Takasawa 2 6,  Kousuke Iba 7,  Atsushi Teramoto 1,  Makoto Osanai 2
Affiliations

Affiliations

  • 1 Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, West 16, South 1, Chuo-ku, Sapporo, 060-8543, Japan.
  • 2 Departments of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
  • 3 Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, West 16, South 1, Chuo-ku, Sapporo, 060-8543, Japan. emrmkt@yahoo.co.jp.
  • 4 Department of Medical Oncology, Sapporo Medical University School of Medicine, Sapporo, Japan.
  • 5 Department of Surgical Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan.
  • 6 Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Asahikawa, Japan.
  • 7 Department of Musculoskeletal Anti-Aging Medicine, Sapporo Medical University, Sapporo, Japan.
PMID: 39625530 DOI: 10.1007/s13577-024-01157-9
Abstract

Myxofibrosarcoma (MFS) is one of the most common soft-tissue sarcomas in elderly patients. Owing to the limited efficacy of chemotherapy and radiotherapy, complete resection is the only available curative treatment. Therefore, developing novel therapies for MFS is important to improve clinical outcomes. Herein, a novel MFS cell line, namely SMU-MFS, was established to better understand the biologic characteristics of MFS and develop new therapies. A tissue sample from the surgically resected tumor tissue of a 56-year-old patient with a tumor was subjected to primary culture. The cell line was established and authenticated by assessing the short tandem repeats of DNA microsatellites. The monolayer cultures of SMU-MFS cells exhibited constant growth, spheroid formation, and invasive capacity. Furthermore, the cells exhibited low chemosensitivity to doxorubicin, eribulin, and pazopanib, which are used to inhibit metastatic progression. In addition, of the four mice inoculated with SMU-MFS cells, tumors developed in two mice after 8 weeks. Altogether, the findings of this study suggest that the SMU-MFS cell line can be a useful tool for investigating MFS development and evaluating novel therapeutic agents.

Keywords

Cell line; Chemotherapy; Local recurrence; Myxofibrosarcoma.

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