2. Academic Validation
  3. Identification of potential substrates of LNX1 in chicken cells

Identification of potential substrates of LNX1 in chicken cells

  • Poult Sci. 2025 Jan;104(1):104633. doi: 10.1016/j.psj.2024.104633.
Yuhong Liu 1,  Qianmei Gao 1,  Qi Zhang 1,  Chen Li 2,  Sha Liu 1,  Meng Su 1,  Danli Song 1,  Guiping Zhao 1,  Qinghe Li 3
Affiliations

Affiliations

  • 1 Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
  • 2 Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
  • 3 Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: liqinghe@caas.cn.
PMID: 39693965 DOI: 10.1016/j.psj.2024.104633
Abstract

Ubiquitination of proteins is involved in numerous life activities. In poultry, several ubiquitin ligases tightly control the innate immune response, imbalance of which may result in autoimmune diseases and seriously impair poultry growth, development, and productivity. LNX1, an E3 ubiquitin Ligase, catalyzes the ubiquitination and subsequent degradation of specific substrate proteins. A cell line with stable knockdown of the LNX1 gene was developed using chicken macrophages (HD11) as the model. The relative quantitative values of each protein were analyzed using 4D-FastDIA quantitative proteomics. This identified 319 proteins with up-regulated expression, a majority of which relate to the immune system. Overall, this study uncovered potential substrate proteins targeted by LNX1 and their associated biological pathways. The findings suggest that LNX1 could be implicated in the regulation of the chicken innate immune system by mediating protein ubiquitination.

Keywords

HD11; Innate Immunity; LNX1; Proteomics; Ubiquitination.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z