2. Academic Validation
  3. Screening and identification of antimicrobial peptides from the gut microbiome of cockroach Blattella germanica

Screening and identification of antimicrobial peptides from the gut microbiome of cockroach Blattella germanica

  • Microbiome. 2024 Dec 21;12(1):272. doi: 10.1186/s40168-024-01985-9.
Sizhe Chen # 1 2 3,  Huitang Qi # 1,  Xingzhuo Zhu # 4,  Tianxiang Liu 5,  Yuting Fan 1,  Qi Su 2 3,  Qiuyu Gong 6,  Cangzhi Jia 7,  Tian Liu 8
Affiliations

Affiliations

  • 1 MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian, Liaoning, 116024, China.
  • 2 Microbiota I-Center (MagIC), Hong Kong SAR, China.
  • 3 The Department of Medicine & Therapeutics, The Chinese University of Hong Kong, ShatinHong Kong SAR, NT, China.
  • 4 Department of Thoracic Surgery, The First Affiliated Hospital of Xiaan Jiaotong University, Xian, 710061, China.
  • 5 School of Science, Dalian Maritime University, Dalian, 116026, China.
  • 6 Department of Thoracic Surgery, The First Affiliated Hospital of Xiaan Jiaotong University, Xian, 710061, China. gongqiuyu@xjtu.edu.cn.
  • 7 School of Science, Dalian Maritime University, Dalian, 116026, China. cangzhijia@dlmu.edu.cn.
  • 8 MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian, Liaoning, 116024, China. tianliu@dlut.edu.cn.
  • # Contributed equally.
PMID: 39709489 DOI: 10.1186/s40168-024-01985-9
Abstract

Background: The overuse of Antibiotics has led to lethal multi-antibiotic-resistant Microorganisms around the globe, with restricted availability of novel Antibiotics. Compared to conventional Antibiotics, evolutionarily originated antimicrobial Peptides (AMPs) are promising alternatives to address these issues. The gut microbiome of Blattella germanica represents a previously untapped resource of naturally evolving AMPs for developing antimicrobial agents.

Results: Using the in-house designed tool "AMPidentifier," AMP candidates were mined from the gut microbiome of B. germanica, and their activities were validated both in vitro and in vivo. Among filtered candidates, AMP1, derived from the symbiotic microorganism Blattabacterium cuenoti, demonstrated broad-spectrum Antibacterial activity, low cytotoxicity towards mammalian cells, and a lack of hemolytic effects. Mechanistic studies revealed that AMP1 rapidly permeates the Bacterial cell and accumulates intracellularly, resulting in a gradual and mild depolarization of the cell membrane during the initial incubation period, suggesting minimal direct impact on membrane integrity. Furthermore, observations from fluorescence microscopy and scanning electron microscopy indicated abnormalities in Bacterial binary fission and compromised cell structure. These findings led to the hypothesis that AMP1 may inhibit Bacterial cell wall synthesis. Furthermore, AMP1 showed potent Antibacterial and wound healing effects in mice, with comparable performances of vancomycin.

Conclusions: This study exemplifies an interdisciplinary approach to screening safe and effective AMPs from natural biological tissues, and our identified AMP 1 holds promising potential for clinical application.

Keywords

Blattella germanica; Antimicrobial peptides; Deep learning tool; Gut microbiome; In vivo activity.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z