2. Academic Validation
  3. Photocontrolled Bionic Micro-Nano Hydrogel System used Novel Functional Strategy for Cell Delivery and Large-Scale Corneal Repair

Photocontrolled Bionic Micro-Nano Hydrogel System used Novel Functional Strategy for Cell Delivery and Large-Scale Corneal Repair

  • Adv Healthc Mater. 2025 Jan 26:e2403444. doi: 10.1002/adhm.202403444.
Mingshan Zhang 1 2,  Shi-Yao Zhang 2 3,  Huiqin Zhang 2 3 4 5,  Youwei Liu 6,  Yipeng Dong 7,  Daobo Han 7,  Le Chang 2 3 5,  Ning Yang 6 8,  Jianguo Tian 1 7,  Yan Wang 2 3 5,  Qing Ye 2 7
Affiliations

Affiliations

  • 1 Institute of Modern Optics, Nankai University, Tianjin, 300350, China.
  • 2 Nankai University Eye Institute, Nankai University, Tianjin, 300071, China.
  • 3 Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin Eye Institute, Tianjin Eye Hospital, Nankai University Affiliated Eye Hospital, Tianjin, 300020, China.
  • 4 Department of Ophthalmology Cangzhou Central Hospital, 16 Xinhua West Road, Cangzhou, Hebei, 061000, China.
  • 5 Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, 300070, China.
  • 6 State Key Laboratory of Separation Membranes and Membrane Processes, National Center for International Joint Research on Separation Membranes, School of Materials Science and Engineering, Tiangong University, Tianjin, 300387, China.
  • 7 Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics, Nankai University, Tianjin, 300071, China.
  • 8 Cangzhou Institute of Tiangong University, Cangzhou, 061000, China.
PMID: 39865725 DOI: 10.1002/adhm.202403444
Abstract

Reproducing the microstructure of the natural cornea remains a significant challenge in achieving the mechanical and biological functionality of artificial corneas. Therefore, the development of cascade structures that mimic the natural extracellular matrix (ECM), achieving both macro-stability and micro-structure, is of critical importance. This study proposes a novel, efficient, and general photo-functionalization strategy for modifying natural biomaterials. Collagen microfibers obtained through electrospinning are functionalized with an active N-Hydroxysuccinimide (NHS) ester, to impart light-curing ability. This approach expands the construction of photo-controllable hydrogel systems beyond conventional single methacrylate (MA) modifications or di-tyrosine bonding, enabling integration with Other biomaterials for comprehensive ECM remodeling. Subsequently, the collagen microfibers are then photo-embedded into gelatin methacryloyl (GelMA) via covalent crosslinking to form a fibrous hydrogel, which supports both structural and functional requirements. In terms of biological functionality, the hydrogel promotes significant inward migration and retention of human corneal fibroblasts (hCFs), replicating ECM-like environments. Furthermore, its excellent burst resistance suggests potential as a bioadhesive. In a rabbit model, the hydrogel achieved effective repair of large-sized (6 mm) corneal defects, facilitates epithelial migration, and maintained long-term stability. This work provides valuable guidance for designing efficient and simplified bioactive Materials for corneal repair and broader tissue engineering applications.

Keywords

electrospinning; fibrous hydrogel; intracellular migration; large‐scale corneal repair; photo functionalization.

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