2. Academic Validation
  3. Shigella OspF blocks rapid p38-dependent priming of the NAIP-NLRC4 inflammasome

Shigella OspF blocks rapid p38-dependent priming of the NAIP-NLRC4 inflammasome

  • bioRxiv. 2025 Feb 2:2025.02.01.636075. doi: 10.1101/2025.02.01.636075.
Elizabeth A Turcotte 1,  Kyungsub Kim 2,  Kevin D Eislmayr 1,  Lisa Goers 2,  Patrick S Mitchell 3 4,  Cammie F Lesser 2 5 6 7,  Russell E Vance 1 8 9 10
Affiliations

Affiliations

  • 1 Division of Immunology & Molecular Medicine, Department of Molecular & Cell Biology, University of California, Berkeley, United States.
  • 2 Department of Microbiology, Harvard Medical School, Boston, United States.
  • 3 Department of Microbiology, University of Washington, Seattle, United States.
  • 4 Howard Hughes Medical Institute, University of Washington, Seattle, United States.
  • 5 Broad Institute of Harvard and MIT, Cambridge, United States.
  • 6 Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Boston, United States.
  • 7 Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, United States.
  • 8 Center for Emerging and Neglected Disease, University of California, Berkeley, United States.
  • 9 Cancer Research Laboratory, University of California, Berkeley, United States.
  • 10 Howard Hughes Medical Institute, University of California, Berkeley, United States.
PMID: 39975412 DOI: 10.1101/2025.02.01.636075
Abstract

The NAIP-NLRC4 inflammasome senses pathogenic bacteria by recognizing the cytosolic presence of Bacterial proteins such as flagellin and type III secretion system (T3SS) subunits. In mice, the NAIP-NLRC4 inflammasome provides robust protection against Bacterial pathogens that infect intestinal epithelial cells, including the gastrointestinal pathogen Shigella flexneri. By contrast, humans are highly susceptible to Shigella, despite the ability of human NAIP-NLRC4 to robustly detect Shigella T3SS proteins. Why the NAIP-NLRC4 inflammasome protects mice but not humans against Shigella Infection remains unclear. We previously found that human THP-1 cells infected with Shigella lose responsiveness to NAIP-NLRC4 stimuli, while retaining sensitivity to Other inflammasome agonists. Using mT3Sf, a "minimal Shigella" system, to express individual secreted Shigella effector proteins, we found that the OspF effector specifically suppresses NAIP-NLRC4-dependent cell death during Infection. OspF was previously characterized as a phosphothreonine lyase that inactivates p38 and ERK MAP kinases. We found that p38 was critical for rapid priming of NAIP-NLRC4 activity, particularly in cells with low NAIP-NLRC4 expression. Overall, our results provide a mechanism by which Shigella evades inflammasome activation in humans, and describe a new mechanism for rapid priming of the NAIP-NLRC4 inflammasome.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z