2. Academic Validation
  3. Centromere protein U mediates the ubiquitination and degradation of RPS3 to facilitate temozolomide resistance in glioblastoma

Centromere protein U mediates the ubiquitination and degradation of RPS3 to facilitate temozolomide resistance in glioblastoma

  • Drug Resist Updat. 2025 May:80:101214. doi: 10.1016/j.drup.2025.101214.
Jinmin Sun 1,  Wenyu Zhao 2,  Lei Zhang 3,  Sicheng Wu 4,  Senrui Xue 4,  Haowei Cao 4,  Biao Xu 4,  Xinmiao Li 2,  Nan Hu 2,  Tao Jiang 5,  Yixin Xu 5,  Zhifei Wang 6,  Chao Zhang 7,  Jing Ren 8
Affiliations

Affiliations

  • 1 Jiangsu Key Laboratory of Brain Disease Bioinformation, Research Center for Biochemistry and Molecular Biology, Xuzhou Medical University, Xuzhou 221004, China; Laboratory of Clinical and Experimental Pathology, Department of Pathology, Xuzhou Medical University, Xuzhou 221004, China.
  • 2 Laboratory of Clinical and Experimental Pathology, Department of Pathology, Xuzhou Medical University, Xuzhou 221004, China.
  • 3 Department of Neurosurgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221004, China.
  • 4 Jiangsu Key Laboratory of Brain Disease Bioinformation, Research Center for Biochemistry and Molecular Biology, Xuzhou Medical University, Xuzhou 221004, China.
  • 5 Department of General Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221004, China.
  • 6 Department of Neurosurgery, The Third Xiangya Hospital, Central South University, Changsha 410013, China. Electronic address: doctorwangzhifei@163.com.
  • 7 Department of Neurosurgery, The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China on Diagnosis and Treatment of Cerebrovascular Disease, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, The Neurosurgery Institute of Guangdong, Province, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Electronic address: czhangsinap@163.com.
  • 8 Jiangsu Key Laboratory of Brain Disease Bioinformation, Research Center for Biochemistry and Molecular Biology, Xuzhou Medical University, Xuzhou 221004, China. Electronic address: renjing@xzhmu.edu.cn.
PMID: 40023134 DOI: 10.1016/j.drup.2025.101214
Abstract

Aims: Temozolomide (TMZ) is the first-line chemotherapeutic agent for glioblastoma (GBM) therapy; however, resistance to TMZ remains a major obstacle in GBM treatment. The aim of this study is to elucidate the mechanisms underlying TMZ resistance and explore how to enhance the sensitivity of GBM to TMZ.

Methods: GBM organoids were generated from patient samples, and organoid-based TMZ sensitivity testing was performed. Transcriptome Sequencing was conducted on GBM organoids, which identified Centromere protein U (CENPU) as a novel key gene mediating TMZ resistance. Histopathological assessments were carried out using immunohistochemistry (IHC) and Hematoxylin and Eosin (HE) staining. Single-cell Sequencing data were utilized to determine the functional states of CENPU in GBM cells. Intracranial and subcutaneous glioma mouse models were constructed to evaluate the effect of CENPU on TMZ sensitivity. The underlying mechanisms were further investigated using immunofluorescence, lentivirus transduction, co-immunoprecipitation, mass spectrometry, alkaline comet assay et al. RESULTS: CENPU was found to be highly expressed in TMZ-resistant GBM organoids and enhanced the TMZ resistance of GBM cells by promoting DNA damage repair. Its abnormal expression correlates with poor clinical outcomes in glioma patients. In vivo studies demonstrated that downregulation of CENPU enhances the sensitivity of GBM to TMZ. Correspondingly, rescue of CENPU expression reversed this effect on TMZ sensitivity in GBM cells. Mechanistically, CENPU cooperates with TRIM5α to promote the ubiquitination and degradation of RPS3 by inducing its polyubiquitination at the K214 residue. This process subsequently activates the ERK1/2 pathway and promotes the expression of E2F1 and RAD51. Consequently, the degradation of RPS3 and upregulation of RAD51 in GBM cells enhance DNA damage repair, thereby contributing to TMZ resistance.

Conclusion: Our study identified CENPU as a novel key gene mediating TMZ resistance and elucidated its molecular mechanisms, providing a new target to overcome TMZ resistance in GBM.

Keywords

CENPU; DNA damage repair; Glioblastoma; RAD51; RPS3; TMZ resistance.

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