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  3. High-performance liquid chromatographic determination of d-/l-epinephrine enantiomer ratio in lidocaine-epinephrine local anesthetics

High-performance liquid chromatographic determination of d-/l-epinephrine enantiomer ratio in lidocaine-epinephrine local anesthetics

  • J Chromatogr. 1985 May 29;325(1):249-54. doi: 10.1016/s0021-9673(00)96025-4.
J F Allgire,  E C Juenge,  C P Damo,  G M Sullivan,  R D Kirchhoefer
PMID: 4019624 DOI: 10.1016/s0021-9673(00)96025-4
Abstract

A procedure for the determination of the ratio of d- to l-epinephrine in lidocaine-epinephrine local anesthetics is described. Epinephrine was isolated via Sep-Pak cartridges and derivatized with 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate; the d- and l-chiral derivatives were separated and measured by high-performance liquid chromatography. About 70 samples of various dosage forms and concentrations from four manufacturers were successfully analyzed by the method.

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