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  3. A rapid method of concentrating proteins in small volumes with high recovery using Sephadex G-25

A rapid method of concentrating proteins in small volumes with high recovery using Sephadex G-25

  • Anal Biochem. 1984 May 1;138(2):451-3. doi: 10.1016/0003-2697(84)90838-8.
A Saul,  M Don
PMID: 6204553 DOI: 10.1016/0003-2697(84)90838-8
Abstract

A method for concentrating small (10 to 500 microliter) volumes of protein solutions by mixing in a microcentrifuge tube with measured volumes of dry Sephadex G-25 is described. The tube is centrifuged, and the protein solution concentrated up to fivefold passes through a small hole in the base of the tube and is collected. Recovery of protein is 90-95%.

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