2. Academic Validation
  3. Immunoenzymatic methods applied to the search for bioactive taxoids from Taxus baccata

Immunoenzymatic methods applied to the search for bioactive taxoids from Taxus baccata

  • J Nat Prod. 1995 Jul;58(7):1015-23. doi: 10.1021/np50121a005.
Y Guo 1,  R Vanhaelen-Fastré,  B Diallo,  M Vanhaelen,  M Jaziri,  J Homes,  R Ottinger
Affiliations

Affiliation

  • 1 Laboratory of Pharmacognosy and Bromatology, Université Libre de Bruxelles, Belgium.
PMID: 7561894 DOI: 10.1021/np50121a005
Abstract

Polyclonal Antibodies raised against 2'-succinyltaxol-bovine serum albumin (BSA) conjugate were used for the immunodetection of bioactive taxoids in chromatographic fractions of the stem bark extract of Taxus baccata. In addition to taxol, cephalomannine, and baccatin III, two taxoids were isolated and their structures were elucidated as 4 alpha,7 beta-diacetoxy-2 alpha,9 alpha-dibenzoxy-5 beta,20-epoxy-10 beta, 13 alpha, 15-trihydroxy-11(15-->1)-abeo-tax-11-ene[5] and taxol C [6] using spectroscopic methods.

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