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  3. Msr-Ratio

Msr-Ratio  (Synonyms: Msr-green)

目录号: HY-D1257 纯度: 99.13%
COA 产品使用指南

Msr-Ratio (Msr-green)是一种比率型甲硫氨酸亚砜还原酶 (methionine sulfoxide reductase) 的荧光探针 (λex=375 nm, λem=550 nm)。Msr-Ratio 可用于监测体外和活细胞中的甲硫氨酸亚砜还原酶活性。

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Msr-Ratio Chemical Structure

Msr-Ratio Chemical Structure

CAS No. : 2290635-22-0

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规格 价格 是否有货 数量
1 mg ¥720
In-stock
5 mg ¥1620
In-stock
10 mg ¥2565
In-stock
25 mg ¥4320
In-stock
50 mg ¥5940
In-stock
100 mg   询价  
200 mg   询价  

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Customer Review

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Msr-Ratio (Msr-green) is a ratiometric fluorescent probe of methionine sulfoxide reductase (λex=375 nm, λem=550 nm). Msr-Ratio is used for monitoring the enzyme activity in vitro and in live cells[1].

体外研究
(In Vitro)

Msr-Ratio is converted to its corresponding sulfide form by methionine sulfoxide reductase. Msr-Ratio displays favorable properties such as a nearly 400-fold fluorescence change, fast response rate (<30 min), large Stokes shift (120 nm), and green emission (550 nm)[1].
There is a very weak background signal in live HL60 cells after the addition of Msr-Ratio. As the Msr-Ratio (10 μM) incubation time increased in cells from 0 to 8 h, the fluorescence signal constantly strengthened[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

310.37

Formula

C18H14O3S

CAS 号
性状

固体

颜色

White to off-white

Emission (Em)

550

Excitation (Ex)

375

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
细胞实验: 

DMSO 中的溶解度 : 100 mg/mL (322.20 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.2220 mL 16.1098 mL 32.2196 mL
5 mM 0.6444 mL 3.2220 mL 6.4439 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2220 mL 16.1098 mL 32.2196 mL 80.5490 mL
5 mM 0.6444 mL 3.2220 mL 6.4439 mL 16.1098 mL
10 mM 0.3222 mL 1.6110 mL 3.2220 mL 8.0549 mL
15 mM 0.2148 mL 1.0740 mL 2.1480 mL 5.3699 mL
20 mM 0.1611 mL 0.8055 mL 1.6110 mL 4.0275 mL
25 mM 0.1289 mL 0.6444 mL 1.2888 mL 3.2220 mL
30 mM 0.1074 mL 0.5370 mL 1.0740 mL 2.6850 mL
40 mM 0.0805 mL 0.4027 mL 0.8055 mL 2.0137 mL
50 mM 0.0644 mL 0.3222 mL 0.6444 mL 1.6110 mL
60 mM 0.0537 mL 0.2685 mL 0.5370 mL 1.3425 mL
80 mM 0.0403 mL 0.2014 mL 0.4027 mL 1.0069 mL
100 mM 0.0322 mL 0.1611 mL 0.3222 mL 0.8055 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Msr-Ratio
目录号:
HY-D1257
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