1. 资源中心
  2. Protocols
  3. 分子生物学专题
  4. 双荧光素酶实验
双荧光素酶实验

双荧光素酶实验(Luciferase, LUC)是一种广泛应用于基因表达调控和细胞信号通路研究的生物技术。该实验利用荧光素酶基因转染到细胞中,通过检测其产生的荧光素酶活性来量化目标基因的表达水平。Luciferase 实验灵敏、高通量,可应用于活体成像和高通量筛选。该技术在基因调控、蛋白交互作用、药物筛选等领域提供了强大的工具,促进了生物医学研究和药物开发。


Dual-luciferase assay (Luciferase, LUC) is a biotechnology widely used in the study of gene expression regulation and cell signaling pathways. This experiment uses luciferase genes to be transfected into cells and the expression level of the target gene is quantified by detecting the luciferase activity produced. Luciferase experiments are sensitive, high-throughput, and can be applied to in vivo imaging and high-throughput screening. This technology provides powerful tools in the fields of gene regulation, protein interaction, drug screening and other fields, and promotes biomedical research and drug development.

相关实验方案

  • 荧光素酶报告基因检测是以荧光素 (luciferin) 为底物来检测萤火虫荧光素酶 (Firefly Luciferase) 活性的一种报告系统,常应用于 miRNA 靶基因验证及启动子转录活性调控等研究。双荧光素酶通常是指萤火虫荧光素酶和海肾荧光素酶。