Southern Blot是一种分子生物学技术，用于检测DNA序列。该方法包括DNA电泳、转移到膜上、DNA固定、DNA探针杂交和检测。Southern Blot常用于确定目标基因的存在、大小、数量和结构。该技术的应用领域涵盖基因组学研究、基因重排的检测、DNA甲基化分析等。尽管在DNA检测领域有着广泛的应用，但Southern Blot的操作相对繁琐，近年来逐渐被更高通量的技术，如PCR和测序等替代。

Southern Blot is a molecular biology technique used to detect DNA sequences. The method includes DNA electrophoresis, transfer to membrane, DNA fixation, DNA probe hybridization and detection. Southern Blot is often used to determine the presence, size, quantity, and structure of target genes. The application fields of this technology include genomics research, detection of gene rearrangements, DNA methylation analysis, etc. Although widely used in the field of DNA detection, the operation of Southern Blot is relatively cumbersome and has been gradually replaced by higher-throughput technologies such as PCR and sequencing in recent years.