蛋白免疫印迹（Western Blotting，WB）是一种用于检测和分析蛋白的分子生物学技术。该方法通过电泳将蛋白分离，然后将其转移到膜上。随后，膜与特异性抗体反应，形成免疫复合物，最后通过化学或荧光检测方法显示目标蛋白。WB 可用于研究蛋白表达水平、鉴定蛋白的分子量、研究蛋白修饰等，是分子生物学和生物医学研究中常用的工具。

Western Blotting (WB) is a molecular biology technique used to detect and analyze proteins. This method separates proteins by electrophoresis and then transfers them to a membrane. The membrane then reacts with specific antibodies to form immune complexes, and finally the target protein is revealed via chemical or fluorescent detection methods. WB can be used to study protein expression levels, identify protein molecular weights, study protein modifications, etc. It is a commonly used tool in molecular biology and biomedical research.