14-3-3 epsilon 蛋白, Mouse (His)

14-3-3 epsilon proteins are adapter proteins in various signaling pathways that bind to phosphoserine or phosphothreonine motifs to regulate chaperone activity.It positively regulates HSF1 nuclear export to the cytoplasm, forming homo- and heterodimers with YWHAZ.14-3-3 epsilon Protein, Mouse (His) is the recombinant mouse-derived 14-3-3 epsilon protein, expressed by E.coli , with N-6*His labeled tag.

14-3-3 epsilon 蛋白作为衔接子参与多种通用和专门信号通路的调节，通过识别磷酸丝氨酸或磷酸苏氨酸基序与众多伴侣结合，从而调节结合伴侣的活性。值得注意的是，它正向调节磷酸化蛋白 HSF1 向细胞质的核输出。它以同二聚体形式存在，还与 YWHAZ 形成异二聚体，并与多种蛋白质相互作用，包括 PKA 磷酸化的 AANAT、磷酸化形式的 ABL1、ARHGEF28、BEX3、CDKN1B、DAPK2 的“Thr-369”磷酸化形式、DENND1A、GAB2、磷酸化的 GRB10、KSR1、NDEL1、PI4KB、TBC1D22A、TBC1D22B、SRPK2 的磷酸化形式、TIAM2、SOS1 的“Ser-1134”和“Ser-1161”磷酸化形式、ZFP36、SLITRK1、磷酸化形式的 HSF1、RIPOR2，KLHL22、CRTC1、CRTC2（可能在“Ser-171”处磷酸化时）、CRTC3（可能在“Ser-162”和/或“Ser-273”处磷酸化时）、ATP2B1、ATP2B3 和 MEFV。这些相互作用凸显了 14-3-3 epsilon 在协调各种细胞过程和信号事件中的多方面作用。

Mouse

E. coli

N-6*His

P62259 (M1-Q255)

22627  [NCBI]

MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ

Approximately 31 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.