40S Ribosomal 蛋白 S3/RPS3 蛋白, Human (His)

40S ribosomal protein S3 (RPS3) is an important component of the small ribosomal subunit and is integral to cellular protein synthesis. In addition to its ribosomal function, RPS3 exhibits endonuclease activity and plays a role in DNA repair. 40S Ribosomal Protein S3/RPS3 Protein, Human (His) is the recombinant human-derived 40S Ribosomal protein S3/RPS3 protein, expressed by E. coli , with C-His labeled tag.

40S 核糖体蛋白 S3 (RPS3) 是小核糖体亚基的组成部分，有助于负责蛋白质合成的细胞机制。除了其核糖体作用外，RPS3 还具有核酸内切酶活性，参与受损 DNA 的修复。它具有广泛的底物特异性，可切割含有改变碱基的 DNA 中的磷酸二酯键，并且与松弛的 DNA 相比，切割超螺旋 DNA 的效率更高。RPS3 对 7,8-二氢-8-氧代鸟嘌呤 (8-oxoG) 表现出很强的亲和力，这是一种由活性氧诱导的常见 DNA 损伤。此外，它还通过刺激碱基切除蛋白（例如 OGG1 和 UNG1）的活性以及促进 APEX1 对磷酸二酯主链的裂解来影响 DNA 修复过程。除了在 DNA 修复中的作用外，RPS3 还参与各种细胞功能，包括作为 NF-kappa-B p65-p50 复合物一部分的转录调节、通过调节微管聚合调节纺锤体形成和有丝分裂过程中的染色体运动，以及诱导细胞凋亡通过与 CASP8 和 E2F1 等关键凋亡因子的相互作用。此外，RPS3 还参与保护 TP53/p53 免受 MDM2 介导的泛素化的影响，并响应过氧化氢暴露而负向调节 DNA 修复。

Human

E. coli

C-His

P23396-1 (A2-A243)

6188  [NCBI]

AVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPTGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA

Approximately 32 kDa

• 
  Greater than 92% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.