1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. AADAC 蛋白, Human (His-SUMO)

AADAC 蛋白, Human (His-SUMO)

目录号: HY-P72067
COA 产品使用指南

AADAC, Human (His-SUMO) 是一种在内质网起作用的酯酶,参与多种药物的水解。 AADAC, Human 过度表达可改变多种血管平滑肌细胞特性并减少鼠心血管疾病。

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规格 价格 是否有货 数量
20 μg ¥1750 In-stock
50 μg ¥3850 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

AADAC, Human (His-SUMO) is an esterase functioning at the endoplasmic reticulum which involved in the hydrolysis of various drugs. AADAC, Human overexpression alters multiple vascular smooth muscle cells properties and decreases murine cardiovascular disease.

研究背景

芳基乙酰胺脱乙酰酶 (AADAC) 是负责水解各种临床药物的主要酶,例如氟他胺、非那西丁、利福霉素、吲地普隆、普拉格雷和酮康唑。AADAC 在人类肝脏和胃肠道中表达。尽管小鼠和大鼠具有多种 Ces1 和 Ces2 亚型,但 AADAC 在动物物种中是保守的,包括小鼠、大鼠、狗和猴子。有报道称,狗CES1和CES2在肠道中的表达水平远低于肝脏中的表达水平。AADAC 过度表达会改变多种血管平滑肌细胞的特性并减少小鼠心血管疾病[1][2]

生物活性

Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is 886.313 pmol/min/µg, as measured under the described conditions.

种属

Human

表达系统

E. coli

标签

N-10*His;N-SUMO

蛋白编号

P22760 (P24-L399)

基因 ID

13  [NCBI]

蛋白结构
N-term
10*His-SUMO
AADAC (P24-L399)
Accession # P22760
C-term
同用名
AAAD_HUMAN; Aada; Aadac; Arylacetamide deacetylase esterase; Arylacetamide deacetylase; CES5A1; DAC
氨基酸序列

PDNVEEPWRMMWINAHLKTIQNLATFVELLGLHHFMDSFKVVGSFDEVPPTSDENVTVTETKFNNILVRVYVPKRKSEALRRGLFYIHGGGWCVGSAALSGYDLLSRWTADRLDAVVVSTNYRLAPKYHFPIQFEDVYNALRWFLRKKVLAKYGVNPERIGISGDSAGGNLAAAVTQQLLDDPDVKIKLKIQSLIYPALQPLDVDLPSYQENSNFLFLSKSLMVRFWSEYFTTDRSLEKAMLSRQHVPVESSHLFKFVNWSSLLPERFIKGHVYNNPNYGSSELAKKYPGFLDVRAAPLLADDNKLRGLPLTYVITCQYDLLRDDGLMYVTRLRNTGVQVTHNHVEDGFHGAFSFLGLKISHRLINQYIEWLKENL

分子量

Approximately 63.1 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

AADAC Protein, Human (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
AADAC Protein, Human (His-SUMO)
目录号:
HY-P72067
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