ABL1 蛋白, Human (sf9, GST)

ABL1 is a non-receptor tyrosine protein kinase that coordinates key cellular processes for growth and survival. It affects cytoskeletal remodeling, cell motility, and receptor endocytosis. ABL1 Protein, Human (sf9, GST) is the recombinant human-derived ABL1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of ABL1 Protein, Human (sf9, GST) is 418 a.a., with molecular weight of ~65 kDa.

ABL1 是一种非受体酪氨酸蛋白激酶，协调对生长和生存至关重要的多种细胞过程。其多方面的作用包括响应细胞外信号协调细胞骨架重塑、调节细胞运动和影响受体内吞作用。ABL1 的影响延伸到自噬、DNA 损伤反应和细胞凋亡，它使这些途径中涉及的关键底物磷酸化。值得注意的是，它通过磷酸化 WASF3 和 ANXA1 等蛋白质来调节肌动蛋白动力学，影响板状伪足形成和细胞迁移等过程。ABL1 是细胞粘附和运动的关键参与者，它可以磷酸化 BCAR1 和 CRK 等调节因子。此外，它还参与受体酪氨酸激酶的调节，包括促进 EGFR 内吞作用。ABL1 的作用范围延伸到氧化应激期间的线粒体功能，介导功能障碍和细胞死亡。此外，它易位到细胞核，有助于 DNA 损伤反应、细胞凋亡和 T 细胞分化的调节。ABL1 广泛的底物库包括参与 DNA 修复和细胞凋亡的蛋白质，展示了其在细胞稳态和对外部刺激的反应中的核心作用。

The specific activity was determined to be >240 nmol/min/mg using synthetic Abl peptide (EAIYAAPFAKKK) as substrate.

Human

Sf9 insect cells

N-GST

P00519-2 (P137-S554)

25  [NCBI]

PVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTS

Approximately 65 kDa

Greater than 80% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM EDTA, 0.5 mM Reduced Glutathione, pH 8.0

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.