ACAT2 蛋白, Rat (His)

ACAT2 Protein is pivotal in cholesterol biosynthesis, intricately contributing to cellular lipid homeostasis regulation. Its involvement underscores its significance in modulating cholesterol levels, highlighting an essential function in cellular physiology. ACAT2 Protein, Rat (His) is the recombinant rat-derived ACAT2 protein, expressed by E. coli , with N-His labeled tag. The total length of ACAT2 Protein, Rat (His) is 397 a.a., with molecular weight of ~43.3 kDa.

ACAT2 蛋白在胆固醇的生物合成途径中发挥着关键作用。作为这一代谢过程不可或缺的组成部分，ACAT2 参与胆固醇的复杂合成，有助于调节和维持细胞脂质稳态。该酶的参与凸显了其在调节胆固醇水平方面的重要性，并强调了其在细胞生理学中的重要功能。

Measured by its ability to convert acetoacetyl-CoA and CoA into acetyl-CoA. The specific activity is 7519.724 pmol/min/µg that incubate at room temperature in kinetic mode for 10 minutes.

Rat

E. coli

N-6*His

Q5XI22 (M1-G397)

308100  [NCBI]

MNAGSDPVVIISAARTAIGSFNGALSTVPVHNLGTTVIKEVLQRAKVAPEEVSEVIFGHVLTAGCGQNPTRQASVGAGIPYSVPAWSCQMICGSGLKAVCLAAQSIAMGDSTIVVAGGMENMSKAPHLAHLRSGVKMGEVPLADSILCDGLTDAFHNYHMGITAENVAKKWQVSREAQDKVAVVSQNRAEHAQKAGHFDKEIVPVHVSSRKGLTEVKIDEFPRHGSNLEAMSKLKPYFLTDGTGTVTPANASGMNDGAAAVVLMKKTEAESRMLKPLAQVVSWSQAGVEPSVMGVGPIPAIKQAVAKAGWSLEDVDVFEINEAFAAVSAAIAKELGLSPEKVNIDGGAIALGHPLGASGCRILVTLLHTLERVGGTRGVAALCIGGGMGIAMCVQR

Approximately 43.3 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.