ACLY 蛋白, Human (His-SUMO)

ACLY Protein, Human (His-SUMO) is an important enzyme in the cholesterol biosynthetic pathway. ACLY produces acetyl-CoA (AcCoA) from mitochondrial citrate for cholesterol and fatty acid biosynthesis.

ATP 柠檬酸裂解酶 (ACLY) 是一种重要的酶，通过从柠檬酸盐生成乙酰辅酶 A 来进行脂肪酸和胆固醇的生物合成，从而将碳水化合物与脂质代谢联系起来。ACLY 是胆固醇生物合成途径中的一种重要酶，位于 3-羟基3-甲基戊二酰辅酶 A 还原酶 (HMGCR)（他汀类药物的靶向）上游。ACLY 从线粒体柠檬酸产生乙酰辅酶 A (AcCoA)，用于胆固醇和脂肪酸的生物合成。ACLY 通过 C 末端（柠檬酸合酶同源域）形成同源四聚体，促进 ACLY 与 CoA 结合并产生 AcCoA^[1]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His;N-SUMO

P53396 (K4-K265)

47  [NCBI]

KAISEQTGKELLYKFICTTSAIQNRFKYARVTPDTDWARLLQDHPWLLSQNLVVKPDQLIKRRGKLGLVGVNLTLDGVKSWLKPRLGQEATVGKATGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNPEDIKKHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGVYVLDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLK

Approximately 45.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.