1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. ACPS 蛋白, Streptococcus pyogenes serotype M28 (Baculovirus, His-Myc)

ACPS 蛋白, Streptococcus pyogenes serotype M28 (Baculovirus, His-Myc)

目录号: HY-P72060
产品使用指南

ACPS 蛋白, Streptococcus pyogenes serotype M28 (Baculovirus, His-Myc),一种 4-磷酸泛酰巯基乙胺转移酶,可激活脂肪酸合酶 (FAS) 系统 FAS-I 和 FAS-II 中存在的两种不同的酰基载体蛋白 (ACP),分别是 ACP-I 结构域和分枝杆菌 ACP-II 蛋白 (ACPM)。

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  • 生物活性

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  • 产品资料

  • 参考文献

描述

ACPS Protein, Streptococcus pyogenes serotype M28 (Baculovirus, His-Myc), a 4-phosphopantetheinyl transferase, activates two distinct acyl carrier proteins (ACPs) that are present in fatty acid synthase (FAS) systems FAS-I and FAS-II, the ACP-I domain and the mycobacterial ACP-II protein (ACPM), respectively[1].

研究背景

酰基载体蛋白合酶 (AcpS) 长度约为 120 个氨基酸残基,呈现出具有生物活性的 α/β 折叠三聚体排列。来自不同物种的 AcpS 及其 ACP 底物之间基于结构的序列比较表明,棒状杆菌亚科的蛋白质表现出高度的序列保守性,形成了 AcpS 和 ACP 的分离亚组。对不同物种的 AcpS 及其 ACP 底物进行的基于序列的结构分析表明,对于棒杆菌科细菌,AcpS、ACP-I 和 ACPM 均表现出高度的序列保守性,这使它们与其他细菌、真菌和寄生虫区分开来[1]

种属

Others

表达系统

Sf9 insect cells

标签

N-10*His;C-Myc

蛋白编号

Q48RM7 (M1-K118)

基因 ID

/

蛋白结构
N-term
10*His
ACPS (M1-K118)
Accession # Q48RM7
Myc
C-term
同用名
acpS; M28_Spy1523Holo-[acyl-carrier-protein] synthase; Holo-ACP synthase; EC 2.7.8.7; 4'-phosphopantetheinyl transferase AcpS
氨基酸序列

MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYKRRLSYLAGRWAGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISISHSGNYVQASVILEDKK

分子量

Approximately 17.1 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
参考文献

ACPS Protein, Streptococcus pyogenes serotype M28 (Baculovirus, His-Myc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ACPS Protein, Streptococcus pyogenes serotype M28 (Baculovirus, His-Myc)
目录号:
HY-P72060
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