1. 重组蛋白
  2. Enzymes & Regulators
  3. Ligases (EC 6)
  4. ACSS2 蛋白, Human (His)

ACSS2 蛋白, Human (His)

目录号: HY-P701923
COA 产品使用指南

ACSS2 蛋白在催化短链脂肪酸合成乙酰辅酶 A 方面发挥着关键作用,乙酸盐是首选底物。尽管乙酸盐得到了有效利用,但 ACSS2 也可以处理丙酸盐,尽管亲和力明显较低。ACSS2 蛋白, Human (His) 是重组的 ACSS2 蛋白,由 E. coli 表达,带有 N-6*His 标签。ACSS2 蛋白, Human (His) 全长 700 个氨基酸。

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规格 价格 是否有货 数量
10 μg ¥3000 In-stock
50 μg ¥8200 In-stock
100 μg   询价  

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描述

The ACSS2 protein plays a key role in catalyzing the synthesis of acetyl-CoA from short-chain fatty acids, with acetate being the preferred substrate. Although acetate is efficiently utilized, ACSS2 can also process propionate, albeit with significantly lower affinity. ACSS2 Protein, Human (His) is the recombinant human-derived ACSS2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of ACSS2 Protein, Human (His) is 700 a.a., .

研究背景

ACSS2(乙酰辅酶A合成酶2)是一种酶,通过催化短链脂肪酸合成乙酰辅酶A,在细胞代谢中发挥着至关重要的作用。乙酸盐是 ACSS2 的首选底物,它能有效地将乙酸盐转化为乙酰辅酶 A。这种酶活性对于各种细胞过程至关重要,包括能量产生、脂质代谢和组蛋白乙酰化。ACSS2 也可以利用丙酸盐,尽管与乙酸盐相比亲和力要低得多。ACSS2 从短链脂肪酸生成乙酰辅酶 A 的能力有助于形成可用于多种代谢途径的乙酰辅酶 A 库,强调了其在细胞能量稳态和代谢信号整合中的重要性。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

Q9NR19 (G2-Q701)

基因 ID

55902

蛋白结构
N-term
6*His
ACSS2 (G2-Q701)
Accession # Q9NR19
C-term
同用名
ACSS2; Acetyl-coenzyme A synthetase; cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acetyl-CoA synthetase 1; AceCS1; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme; Propionate--CoA ligase
分子量

80.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 50 mM HEPES (pH 7.0), 200 mM NaCl, 20% glycerol, 1 mM DTT.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

Please use rapid thawing with running water to thaw the protein.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

ACSS2 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ACSS2 Protein, Human (His)
目录号:
HY-P701923
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