INHBB 蛋白, Human (HEK293, His)

The INHBB protein complexly controls pituitary function, regulating follicle-stimulating hormone and activin. Its critical role extends to a variety of physiological processes, including hormone secretion, cell development, insulin release, and bone growth. INHBB Protein, Human (HEK293, His) is the recombinant human-derived INHBB protein, expressed by HEK293 , with C-10*His labeled tag. The total length of INHBB Protein, Human (HEK293, His) is 379 a.a., with molecular weight of ~17.52 & 40.53 & 53.75 kDa, respectively.

INHBB 蛋白在垂体功能的复杂协调中发挥着至关重要的作用，有助于抑制和激活促卵泡素分泌以及激活素的双重调节动力学。抑制素和激活素的深远影响，以INHBB为关键成分，延伸到一系列生理过程，包括下丘脑和垂体激素分泌、性腺激素分泌、生殖细胞发育和成熟、红细胞分化、胰岛素分泌、神经细胞存活、胚胎轴向发育和骨骼生长，取决于其独特的亚基组成。值得注意的是，以抑制素 A 和抑制素 B 为代表的抑制素作为与激活素功能相反的对应物出现。在结构上，INHBB 以二聚体构型存在，通过一个或多个二硫键错综复杂地连接，代表 β-B 亚基的同二聚体。激活素（包括激活素 A、激活素 B 和激活素 AB）内细微的多样性进一步强调了它们独特的亚基组成及其与调节蛋白（如 FST 和 FSTL3）的相互作用。这种错综复杂的相互作用凸显了 INHBB 在微调管理多种生理功能的综合调控网络中的核心作用。

Recombinant human Activin B (1  ng/mL; 48 hours) protein incubation with the normal fibroblasts (NFBs), the results show  increase expressions of Activin B, Smad2/3, TGF-β1, and endothelin-1 (ET-1) compared with the untreated NFBs. In addition, the levels of thrombospondin-1 (Tsp-1) and Oncostatin M (OSM) are significantly lower than those of the control group^[1]. Recombinant human Activin B (5  ng/mL; 24 hours) significantly decreases E-cadherin expression in both a time- and concentration-dependent manner in KLE and HEC-5 cell lines. The suppressive effects of Activin B on E-cadherin are mediated by MEK-ERK1/2-induced production of the transcription factor SNAIL^[3].

Recombinant human Activin B (5, 1 or 5  ng/mL; dorsal skin; 3 times a day for 17 days) efficiently induces the initiation of hair follicles in the skin of both cultured and neonatal mice and to promote the development of hair follicles in neonatal mouse skin. Moreover, activin-B-treated hair follicles were observed to enter the anagen stage from the telogen stage and to remain in the anagen stage^[5].

1.Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED₅₀ this effect is 0.6762 ng/mL, corresponding to a specific activity is 1.48×10⁶ units/mg. (Note: Protein mainly exists in propeptide form, which means it doesn't have the biological activity of Activin B.)
2.Measured by its binding ability in a functional ELISA.Immobilized human INHBB-His at 10 μg/mL (100 μl/well) can bind biotinylated mouse FLRG-His with a linear range of 0.031-0.125 μg/mL.
3.Immobilized Human INHBB at 10 μg/mL (100 μL/well) can bind Biotinylated FLRG protein. The ED₅₀ for this effect is 156.2 ng/mL.

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  Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED₅₀ this effect is 0.6762 ng/mL, corresponding to a specific activity is 1.48×10⁶ units/mg.

Human

HEK293

C-10*His

P09529/NP_002184.2 (S29-A407)

3625  [NCBI]

SPTPPPTPAAPPPPPPPGSPGGSQDTCTSCGGFRRPEELGRVDGDFLEAVKRHILSRLQMRGRPNITHAVPKAAMVTALRKLHAGKVREDGRVEIPHLDGHASPGADGQERVSEIISFAETDGLASSRVRLYFFISNEGNQNLFVVQASLWLYLKLLPYVLEKGSRRKVRVKVYFQEQGHGDRWNMVEKRVDLKRSGWHTFPLTEAIQALFERGERRLNLDVQCDSCQELAVVPVFVDPGEESHRPFVVVQARLGDSRHRIRKRGLECDGRTNLCCRQQFFIDFRLIGWNDWIIAPTGYYGNYCEGSCPAYLAGVPGSASSFHTAVVNQYRMRGLNPGTVNSCCIPTKLSTMSMLYFDDEYNIVKRDVPNMIVEECGCA

Approximately 17.52&40.53&53.75 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Shi-Kang Deng, et al. Activin B signaling may promote the conversion of normal fibroblasts to scar fibroblasts. Medicine (Baltimore). 2020 Jun 12;99(24):e20253.  [Content Brief]

  [2]. J M Young, et al. Activin B is produced early in antral follicular development and suppresses thecal androgen production. Reproduction. 2012 May;143(5):637-50.  [Content Brief]

  [3]. Siyuan Xiong, et al. Activin B promotes endometrial cancer cell migration by down-regulating E-cadherin via SMAD-independent MEK-ERK1/2-SNAIL signaling. Oncotarget. 2016 Jun 28;7(26):40060-40072.  [Content Brief]

  [4]. Sabine Gravelsina, et al. Potential of Activin B as a Clinical Biomarker in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS). Biomolecules. 2021 Aug 11;11(8):1189.  [Content Brief]

  [5]. Qin Jia, et al. Activin B promotes initiation and development of hair follicles in mice. Cells Tissues Organs. 2013;198(4):318-26.  [Content Brief]