ACVR2B 蛋白, Human (HEK293, Fc)

ACVR2B is a type II member of the TGF-β family of receptor Serine/Threonine kinases. ACVR2B binds to activins and growth differentiation factors (GDF), which in turn activate type I receptors, activating downstream molecule SMAD2/3^[1]. ACVR2B Protein, Human (HEK293, Fc) is produced in HEK293 cells with a C-Terminal Fc-tag. It consists of 134amino acids (M1-T134).

2B 型激活素受体 (ACVR2B)，也称为 ActR-IIB 和 MGC116908，是一种 II 型激活素受体。I 型受体对于信号传导至关重要；结合配体和表达 I 型受体需要 II 型受体^[1]。
不同物种的 ACVR2B 蛋白的氨基酸序列非常稳定，这导致结论是，在进化过程中，ACVR2B仅发生了轻微改变，无论是在人类还是动物中，其功能都是相似的。
激活素和生长分化因子（GDF）与ACVR2B结合，进而激活类型I 受体，如激活素受体样激酶 (ALK) ALK4 和 ALK5，激活下游分子 SMAD2/3。SMADS 调节许多肌原基因，例如 myoD、肌细胞生成素和 Myf5，这些基因参与细胞肥大、增殖或分化。非经典 ACVR2B 通路也被证明可以调节 MAP 激酶。ACVR2B 阻断肌生长抑制素、其密切同源物 GDF11 以及激活素 A、激活素 B 和 BMP10^[1] 的信号传导。激活素 A 主要与 1 型受体 ALK4 或 ALK7 结合，与 ACVR2A 或 ACVR2B 形成复合物，从而激活 SMAD2 或 SMAD3^[2]。
在骨髓瘤细胞中，BMP-6- 和 BMP激活素 A 处理可抑制 -9 通过 ACVR2A/ACVR2B/ALK2 诱导的 SMAD1/5/8 激活^[2]。ACVR2B 已被证明可以在肿瘤宿主中保留肌肉质量并延长生存时间，并在成骨不全和肌营养不良模型中增加骨量^[3]。

Recombinant human ACVR2B (5 μg/mL) inhibits the effects of BMP-9 on INA-6 and IH-1 cells^[2].

1.Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED₅₀ for this effect is 0.02-0.3496 µg/mL in the presence of 10 ng/mL recombinant Activin A.
2.Measured by its binding ability in a functional ELISA. Immobilized Inhibin Human, Mouse, Rat, Cynomolgus, Rhesus Inhibin beta A/Activin A at 2 μg/mL (100 μl/well) can bind Human ACVR2B hFc, the EC₅₀ of Human ACVR2B hFc is 12-60 ng/mL.

• 
  Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.03309 μg/mL in the presence of 15 ng/mL of Recombinant Human BMP‑4, corresponding to a specific activity is 3.022×10⁴ units/mg.

Human

HEK293

C-hFc

Q13705 (S19-T134)

93  [NCBI]

SGRGEAETRECIYYNANWELERTNQSGLERCEGEQDKRLHCYASWRNSSGTIELVKKGCWLDDFNCYDRQECVATEENPQVYFCCCEGNFCNERFTHLPEAGGPEVTYEPPPTAPT

Approximately 55-70 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Johanna Magga, et al. Systemic Blockade of ACVR2B Ligands Protects Myocardium from Acute Ischemia-Reperfusion Injury. Mol Ther. 2019 Mar 6;27(3):600-610.  [Content Brief]

  [2]. Oddrun Elise Olsen, et al. Activin A inhibits BMP-signaling by binding ACVR2A and ACVR2B. Cell Commun Signal. 2015 Jun 6;13:27.  [Content Brief]

  [3]. Rafael Barreto, et al. ACVR2B/Fc counteracts chemotherapy-induced loss of muscle and bone mass. Sci Rep. 2017 Oct 31;7(1):14470.  [Content Brief]