1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) ADAMs/ADAMTSs
  4. ADAM9
  5. ADAM9 蛋白, Mouse (HEK293, His)

ADAM9 蛋白, Mouse (HEK293, His)

目录号: HY-P75531
产品使用指南

ADAM9蛋白是一种金属蛋白酶,在肿瘤发生和血管生成中发挥重要作用。它裂解 TEK、KDR、EPHB4、CD40、VCAM1 和 CDH5,调节癌症相关信号通路和血管形成。ADAM9 蛋白, Mouse (HEK293, His) 是重组的 ADAM9 蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

ADAM9 protein is a metalloprotease that plays an important role in tumorigenesis and angiogenesis. It cleaves TEK, KDR, EPHB4, CD40, VCAM1 and CDH5, regulating cancer-related signaling pathways and blood vessel formation. ADAM9 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ADAM9 protein, expressed by HEK293 , with C-His labeled tag.

研究背景

ADAM9 蛋白是一种金属蛋白酶,参与与肿瘤发生和血管生成相关的关键细胞过程。这种多面酶已知可裂解和释放各种分子,包括 TEK、KDR、EPHB4、CD40、VCAM1 和 CDH5,突出显示其参与调节与癌症进展和血管形成相关的关键信号通路。除了在蛋白水解事件中的作用之外,ADAM9 还被认为在介导细胞-细胞和细胞-基质相互作用中发挥作用,从而可能通过与整合素的相互作用来调节细胞运动。这种多样化的分子相互作用强调了 ADAM9 在影响细胞行为及其对病理过程的潜在影响方面的重要性。

种属

Mouse

表达系统

HEK293

标签

C-10*His

蛋白编号

Q61072 (G30-D697)

基因 ID
蛋白结构
N-term
ADAM9 (M1-D697)
Accession # Q61072
His
C-term
同用名
Disintegrin and metalloproteinase domain-containing protein 9; Meltrin-gamma; ADAM 9
氨基酸序列

GRPDLEQTVHLSSYEIITPWRLTRERREALGPSSQQISYVIQAQGKQHIIHLERNTDLLPNDFVVYTYDKEGSLLSDHPNVQSHCHYRGYVEGVQNSAVAVSACFGLRGLLHLENASFGIEPLHNSSHFEHIFYPMDGIHQEPLRCGVSNRDTEKEGTQGDEEEHPSVTQLLRRRRAVLPQTRYVELFIVVDKERYDMMGRNQTAVREEMIRLANYLDSMYIMLNIRIVLVGLEIWTDRNPINIIGGAGDVLGNFVQWREKFLITRWRHDSAQLVLKKGFGGTAGMAFVGTVCSRSHAGGINVFGQITVETFASIVAHELGHNLGMNHDDGRECFCGAKSCIMNSGASGSRNFSSCSAEDFEKLTLNKGGSCLLNIPKPDEAYSAPSCGNKLVDPGEECDCGTAKECEVDPCCEGSTCKLKSFAECAYGDCCKDCQFLPGGSMCRGKTSECDVPEYCNGSSQFCPPDVFIQNGYPCQNSKAYCYNGMCQYYDAQCQVIFGSKAKAAPRDCFIEVNSKGDRFGNCGFSGSEYKKCATGNALCGKLQCENVQDMPVFGIVPAIIQTPSRGTKCWGVDFQLGSDVPDPGMVNEGTKCDAGKICRNFQCVNASVLNYDCDIQGKCHGHGVCNSNKNCHCEDGWAPPHCDTKGYGGSVDSGPTYNAKSTALRD

分子量

Approximately 74.9 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ADAM9 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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