Adenylate Kinase 1/AK1 蛋白, Rat (His)

The adenylate kinase 1 (AK1) protein plays a crucial role in cellular energy homeostasis by maintaining the balance of adenylate nucleotides by catalyzing the reversible transfer of terminal phosphate groups between ATP and AMP.Adenylate Kinase 1/AK1 Protein, Rat (His) is the recombinant rat-derived Adenylate Kinase 1/AK1 protein, expressed by E.coli , with N-His labeled tag.

腺苷酸激酶 1 (AK1) 是一种多功能酶，可催化 ATP 和 AMP 之间末端磷酸基团的可逆转移，这是细胞能量稳态的关键步骤。此外，AK1 还具有核苷二磷酸激酶活性，可使用 ATP 或 GTP 作为磷酸盐供体，促进从相应的二磷酸底物生成各种核苷三磷酸（ATP、CTP、GTP、UTP、dATP、dCTP、dGTP 和 dTTP）。这种酶的灵活性凸显了其在核苷酸生物合成中的重要作用。此外，AK1 对从二磷酸硫胺素 (ThDP) 和 ADP 合成三磷酸硫胺素 (ThTP) 的催化速率较低，表明其参与硫胺素代谢。AK1 的多方面功能强调了其在维持细胞能量水平和参与多种核苷酸和辅因子生物合成途径中的重要性。参考资料：所提供的信息基于截至 2022 年 1 月的一般知识，未引用具体参考资料。

Specific activity is 933.598 units/mg. One unit will convert ATP + AMP to 2.0 µmoles of ADP per minute at pH 7.5 at 37 °C.

Rat

E. coli

N-6*His

P39069 (M1-K194)

24183  [NCBI]

MEDKLKKAKIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRAEVSSGSSRGKMLSSIMEKGELVPLETVLDMLRDAMLAKVDSSNGFLIDGYPREVKQGEEFERKIAQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVISFYDKRGIVRKVNAEGSVDTVFSQVCTYLDSLK

Approximately 23 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of PBS, 100 mM arginine, pH 7.1, 20% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.