Adiponectin/Acrp30 蛋白, Human

Adiponectin/Acrp30 Protein, Human could increase cell sensitivity to insulin and can be used as a potential protein for treating diabetic tendinopathy.

重组人脂联素可用于受损动脉并减轻血管炎症反应。据报道，生理浓度的重组人脂联素可抑制肿瘤坏死因子-α(TNF-α) 诱导的内皮粘附分子表达、巨噬细胞向泡沫细胞的转化以及巨噬细胞中 TNF-α 的表达^{[1]。重组人脂联素可作为治疗糖尿病肌腱病的潜在蛋白质，在体外促进肌腱细胞祖细胞增殖和肌腱分化。体外[2]}。

The ED₅₀ is <5 μg/mL as measured by M1 cells, corresponding to a specific activity of >2.0 × 10² units/mg.

Human

E. coli

Tag Free

Q15848 (E19-N244)

9370  [NCBI]

METTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN

Approximately 24.7 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Kumada M, et al. Adiponectin specifically increased tissue inhibitor of metalloproteinase-1 through interleukin-10 expression in human macrophages. Circulation. 2004 May 4;109(17):2046-9.  [Content Brief]

  [2]. Rothan HA, et al. Recombinant human adiponectin as a potential protein for treating diabetic tendinopathy promotes tenocyte progenitor cells proliferation and tenogenic differentiation in vitro. Int J Med Sci. 2013 Nov 27;10(13):1899-906.  [Content Brief]