ADRB2 蛋白, Human (Cell-Free, His, SUMO)

ADRB2 protein is an important member of the β-adrenergic receptor family and mediates catecholamine-induced adenylyl cyclase activation through G protein action. It has 30 times greater affinity for epinephrine and plays a crucial role in cellular responses to sympathetic nervous system stimulation. ADRB2 Protein, Human (Cell-Free, His, SUMO) is the recombinant human-derived ADRB2 protein, expressed by E. coli Cell-free , with N-6*His, N-SUMO labeled tag and G16R, E27Q mutation. The total length of ADRB2 Protein, Human (Cell-Free, His, SUMO) is 413 a.a., with molecular weight of 65 kDa.

ADRB2 蛋白属于 β-肾上腺素能受体家族，在通过 G 蛋白作用介导儿茶酚胺诱导的腺苷酸环化酶激活中发挥着关键作用。ADRB2 对肾上腺素的亲和力比去甲肾上腺素高约 30 倍，在细胞对交感神经系统刺激的反应中发挥着关键作用。该蛋白参与多种分子相互作用，与 NHERF1、GPRASP1、ARRB1、ARRB2、SRC、USP20、USP33、VHL、EGLN3、SNX27、CNIH4、ARRDC3、NEDD4 和 MARCHF2 结合。这些相互作用有助于 ADRB2 功能的复杂调节，包括其泛素化、降解和回收过程，强调了其在调节细胞对肾上腺素信号传导反应中的重要性。

Human

E. coli Cell-free

N-6*His;N-SUMO

P07550 (M1-L413, G16R, E27Q)

154

MGQPGNGSAFLLAPNRSHAPDHDVTQQRDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSQGRNCSTNDSLL

65 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% FOS12, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.