AG-2 蛋白, Human (HEK293, His, solution)

AG-2 Protein, Human (HEK293, His, solution) is human recombinant AG-2 with a N-terminal His tag. AG-2 Protein, Human (HEK293, His) is expressed in HEK293 cells.

AG-2（前梯度 2）是非洲爪蟾水泥腺蛋白的人类直系同源物，也是蛋白质二硫键异构酶 (PDI) 家族的成员。AG-2在恶性癌症类型中高表达和分泌，包括乳腺癌、胰腺癌、胶质母细胞瘤、前列腺癌和转移性结直肠癌。AGR2 也是一种促癌蛋白，可刺激 EGFR 配体双调蛋白、调节 p53 信号传导并与 AAA+ 蛋白 Reptin 相互作用。过表达的 AG-2 可稳定肿瘤细胞中的 HIF-1α^[1][2]。

Human

HEK293

C-6*His

O95994 (R21-L175)

10551  [NCBI]

RDTTVKPGAKKDTKDSRPKLPQTLSRGWGDQLIWTQTYEEALYKSKTSNKPLMIIHHLDECPHSQALKKVFAENKEIQKLAEQFVLLNLVYETTDKHLSPDGQYVPRIMFVDPSLTVRADITGRYSNRLYAYEPADTALLLDNMKKALKLLKTELHHHHHH

Approximately 18.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris, 200 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Pan F, et al. Anterior gradient 2 as a supervisory marker for tumor vessel normalization induced by anti-angiogenic treatment. Oncol Lett. 2018 Sep;16(3):3083-3091.  [Content Brief]

  [2]. Luu TT, et al. Overexpression of AGR2 Is Associated With Drug Resistance in Mutant Non-small Cell Lung Cancers. Anticancer Res. 2020 Apr;40(4):1855-1866.  [Content Brief]