AGA/Aspartylglucosaminidase 蛋白, Human (HEK293, His)

The AGA (aspartate glucosaminidase) protein plays a vital role in glycoprotein processing as an enzyme by cleaving the GlcNAc-Asn bond that connects the oligosaccharide to asparagine in the peptide chain of the glycoprotein. connect them. This cleavage represents the specificity of AGA in removing N-linked glycan structures from glycoproteins, a process necessary for the maturation and modification of these proteins. AGA/Aspartylglucosaminidase Protein, Human (HEK293, His) is the recombinant human-derived AGA/Aspartylglucosaminidase protein, expressed by HEK293 , with C-10*His labeled tag. The total length of AGA/Aspartylglucosaminidase Protein, Human (HEK293, His) is 323 a.a., with molecular weight of ~47 & 29 & 23 & 20 kDa, respectively.

天冬氨葡萄糖胺酶 (AGA) 是一种酶，通过裂解 GlcNAc-Asn 键，在糖蛋白代谢中发挥至关重要的作用，该键将寡糖与天冬酰胺连接的糖蛋白的肽部分连接起来。这种特定的酶活性使 AGA 能够参与糖蛋白的降解和加工，有助于这些分子在细胞内的回收和周转。GlcNAc-Asn 键的断裂是糖蛋白水解的关键步骤，促进寡糖和肽的释放。虽然需要进一步研究来充分了解其生理意义，但 AGA 在糖蛋白分解代谢中的作用强调了其在与蛋白质周转和再循环相关的细胞过程中的重要性（参考：提供的信息）。

Measured by its ability to hydrolyze the AspAMC and the specific activity is >300 pmol/min/μg.

Human

HEK293

C-10*His

P20933 (S24-I346)

175  [NCBI]

SSPLPLVVNTWPFKNATEAAWRALASGGSALDAVESGCAMCEREQCDGSVGFGGSPDELGETTLDAMIMDGTTMDVGAVGDLRRIKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFINEDLSTSASQALHSDWLARNCQPNYWRNVIPDPSKYCGPYKPPGILKQDIPIHKETEDDRGHDTIGMVVIHKTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQAVEYMRRGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACNKLSTFTQFSFMVYNSEKNQPTEEKVDCI

Approximately 47&29&23&20 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.