AGER 蛋白, Human (HEK293, His)

AGER Protein, Human (HEK293, His) is human recombinant AGER with a His tag at the C-terminus. AGER Protein, Human (HEK293, His) is expressed by mammalian expression system and the target gene encoding Ala23-Ala344 is expressed.

AGER（高级糖基化终产物特异性受体）是细胞表面分子免疫球蛋白超家族的成员，是一种跨膜信号转导受体，具有许多配体，包括可以启动和维持免疫反应的警报素。AGER 天然以两种形式存在，即全长膜结合形式和截短（可溶）形式。人类 AGER 是一个高度多态性的基因，其位于 6p21.3 染色体上的基因座有超过 190 个 SNP。AGER 与多种配体和多种信号通路相互作用，例如由高迁移率族盒 1 (HMGB1) 蛋白（AGER 的非经典配体）激活的信号通路。在动脉粥样硬化等某些疾病中，AGER 是急性和慢性血管炎症的介质，尤其是糖尿病的并发症^[1][2]。

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human AGER at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA). The ED₅₀ for this effect is 0.9828 μg/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human AGER at 5 µg/mL (100 µL/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA) .The ED₅₀ for this effect is 0.9828 μg/mL.

Human

HEK293

C-6*His

Q15109 (A23-A344)

177  [NCBI]

AQNITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGTLALA

Approximately 48-60 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Biros E, et al. Association between the Advanced Glycosylation End Product-Specific Receptor Gene and Cardiovascular Death in Older Men. PLoS One. 2015 Jul 30;10(7):e0134475.  [Content Brief]

  [2]. Zee RY, et al. Polymorphisms in the advanced glycosylation end product-specific receptor gene and risk of incident myocardial infarction or ischemic stroke. Stroke. 2006 Jul;37(7):1686-90.  [Content Brief]