AKR1A1 蛋白, Human (His)

The AKR1A1 protein uses NADPH to reduce various carbonyl compounds to alcohols. AKR1A1 Protein, Human (His) is the recombinant human-derived AKR1A1 protein, expressed by E. coli , with N-His labeled tag. The total length of AKR1A1 Protein, Human (His) is 325 a.a., with molecular weight of ~39 KDa.

AKR1A1 是一种多功能酶，可催化多种含羰基化合物依赖 NADPH 还原为其各自的醇。AKR1A1 对内源代谢物（如芳香族和脂肪族醛、酮、单糖和胆汁酸）表现出酶活性，并表现出对带负电荷的底物（如葡萄糖醛酸和琥珀半醛）的偏好。作为一种解毒酶，它在减少有毒醛方面发挥着至关重要的作用，包括甲基乙二醛和3-脱氧葡萄糖醛酮，这些醛在高血糖条件下会积累并发挥细胞毒性作用。此外，AKR1A1 有助于脂质衍生醛（例如丙烯醛）的解毒。该酶参与致癌物（包括多环芳烃反式二氢二醇）的激活，并参与各种异生物质和药物的代谢，例如蒽环类阿霉素（DOX）和柔红霉素（DAUN）。值得注意的是，AKR1A1 对类维生素A 不表现出还原酶活性。这种多样化的底物特异性强调了其在细胞解毒过程中的关键作用及其在治疗干预中的潜在相关性。

Measured by its ability to Reduction of ketone. The specific activity is 2227.65 pmol/min/μg, as measured under the described conditions.

Human

E. coli

N-His

P14550 (M1-Y325)

10327  [NCBI]

Approximately 39 kDa.

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.